Distinct phenotypes of antigen-selected CD8 T cells emerge at different stages of an in vivo immune response.

Details

Serval ID
serval:BIB_63BC54857D01
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Distinct phenotypes of antigen-selected CD8 T cells emerge at different stages of an in vivo immune response.
Journal
Journal of immunology
Author(s)
Walker P.R., Ohteki T., Lopez J.A., MacDonald H.R., Maryanski J.L.
ISSN
0022-1767
Publication state
Published
Issued date
1995
Peer-reviewed
Oui
Volume
155
Number
7
Pages
3443-3452
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
We have previously described a unique system for identifying Ag-selected CD8 T cells during an in vivo response in normal mice. In this system, lymphocytes isolated from DBA/2 mice injected i.p. with HLA-CW3 transfected syngeneic (H-2d) P815 cells show a remarkable expansion of CD8 cells that utilize TCR expressing the V beta 10 gene segment and additional structural features characteristic of Kd-restricted CW3-specific CTL clones. We have now taken advantage of this system to characterize the surface phenotype of CD8 cells selected by Ag in vivo. We observed several distinct phenotypes at different stages of the response. At the peak of the response, Ag-selected cells were low in CD62L and CD45RB expression but displayed high levels of CD44. In addition, there was a partial down-regulation of CD8 and TCR. Cells of this phenotype were present in lymphoid tissues for several mo after immunization. Much later in the response, Ag-selected cells expressed higher levels of CD8 and TCR. Moreover, a distinct subset of these long-term immune cells emerged that now expressed CD62L and CD45RB. Analysis of CD8 cells from different tissues also revealed certain differences, particularly in TCR and co-receptor levels from liver-derived cells compared with circulating cells at the peak of the response. Our findings suggest that the function of Ag-selected CD8 cells may be regulated over time and according to location by subtle changes in cell-surface phenotype.
Keywords
Animals, Antigen Presentation, CD8-Positive T-Lymphocytes/immunology, Female, Immunity, Cellular, Immunophenotyping, Lymphoid Tissue/immunology, Mice, Organ Specificity, T-Lymphocyte Subsets
Pubmed
Web of science
Create date
11/02/2010 14:55
Last modification date
20/08/2019 14:20
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