Telomerase activity and hTERT mRNA expression can be heterogeneous and does not correlate with telomere length in soft tissue sarcomas
Details
Serval ID
serval:BIB_63942EA7758C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Telomerase activity and hTERT mRNA expression can be heterogeneous and does not correlate with telomere length in soft tissue sarcomas
Journal
International Journal of Cancer
ISSN
0020-7136 (Print)
Publication state
Published
Issued date
2002
Volume
98
Number
6
Pages
851-856
Notes
PT - Journal Article PT - Research Support, Non-U.S. Gov't
Abstract
In a previous study, we showed that telomerase activity (TA) and human telomerase reverse transcriptase (hTERT) mRNA expression were undetectable in benign mesenchymal lesions and low-grade soft tissue sarcomas (STSs), but detectable in about 50% of intermediate-/high-grade STSs. We wondered if this lack of TA or hTERT mRNA expression could be related to the tumor sample examined and if there was a relationship between the former 2 parameters and telomere length. Two separate tumor samples from 37 STSs were examined for telomerase activity, using the telomerase repeat amplification protocol (TRAP) assay and for hTERT mRNA expression, using RT-PCR. Telomere length was determined in each tumor sample, using the terminal restriction fragments (TRF) technique. Significant variations in telomere length, TA and hTERT mRNA expression between 2 samples of the same tumor were observed in 27%, 11% and 27% of STSs, respectively. Telomere length did not correlate with TA or hTERT mRNA expression. Despite great intratumoral heterogeneity in telomere length, short and long telomeres were more often seen in the low/intermediate-grade and high-grade STS categories, respectively. Few STSs that showed a TRF pattern suggestive of alternative lengthening of telomeres (ALT) may contain ALT subpopulations
Keywords
Adolescent/Adult/Aged/Aged,80 and over/Child/Child,Preschool/DNA-Binding Proteins/Gene Expression Regulation,Neoplastic/Humans/Immunoenzyme Techniques/Infant/Middle Aged/RNA,Messenger/metabolism/Reverse Transcriptase Polymerase Chain Reaction/Sarcoma/enzymology/genetics/Pathology/Soft Tissue Neoplasms/Telomerase/Telomere/chemistry/Transcription,Genetic
Pubmed
Web of science
Open Access
Yes
Create date
29/01/2008 18:35
Last modification date
20/08/2019 14:20