Direct induction of microtubule branching by microtubule nucleation factor SSNA1.

Details

Serval ID
serval:BIB_63896D903CD5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Direct induction of microtubule branching by microtubule nucleation factor SSNA1.
Journal
Nature cell biology
Author(s)
Basnet N., Nedozralova H., Crevenna A.H., Bodakuntla S., Schlichthaerle T., Taschner M., Cardone G., Janke C., Jungmann R., Magiera M.M., Biertümpfel C., Mizuno N.
ISSN
1476-4679 (Electronic)
ISSN-L
1465-7392
Publication state
Published
Issued date
10/2018
Peer-reviewed
Oui
Volume
20
Number
10
Pages
1172-1180
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Microtubules are central elements of the eukaryotic cytoskeleton that often function as part of branched networks. Current models for branching include nucleation of new microtubules from severed microtubule seeds or from γ-tubulin recruited to the side of a pre-existing microtubule. Here, we found that microtubules can be directly remodelled into branched structures by the microtubule-remodelling factor SSNA1 (also known as NA14 or DIP13). The branching activity of SSNA1 relies on its ability to self-assemble into fibrils in a head-to-tail fashion. SSNA1 fibrils guide protofilaments of a microtubule to split apart to form daughter microtubules. We further found that SSNA1 localizes at axon branching sites and has a key role in neuronal development. SSNA1 mutants that abolish microtubule branching in vitro also fail to promote axon development and branching when overexpressed in neurons. We have, therefore, discovered a mechanism for microtubule branching and implicated its role in neuronal development.
Keywords
Animals, Autoantigens/genetics, Autoantigens/metabolism, Autoantigens/ultrastructure, Axons/metabolism, Cells, Cultured, Cryoelectron Microscopy, Cytoskeleton/metabolism, Hippocampus/cytology, Mice, Microtubules/chemistry, Microtubules/metabolism, Microtubules/ultrastructure, Mutation, Neurons/metabolism, Nuclear Proteins/genetics, Nuclear Proteins/metabolism, Nuclear Proteins/ultrastructure
Pubmed
Web of science
Create date
15/10/2018 16:41
Last modification date
20/08/2019 14:20
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