A novel family of dehydrin-like proteins is involved in stress response in the human fungal pathogen Aspergillus fumigatus.

Details

Ressource 1Download: BIB_636F640E2C5A.P001.pdf (2320.06 [Ko])
State: Public
Version: author
Serval ID
serval:BIB_636F640E2C5A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A novel family of dehydrin-like proteins is involved in stress response in the human fungal pathogen Aspergillus fumigatus.
Journal
Molecular Biology of the Cell
Author(s)
Hoi J.W., Lamarre C., Beau R., Meneau I., Berepiki A., Barre A., Mellado E., Read N.D., Latgé J.P.
ISSN
1939-4586 (Electronic)
ISSN-L
1059-1524
Publication state
Published
Issued date
2011
Volume
22
Number
11
Pages
1896-1906
Language
english
Abstract
 During a search for genes controlling conidial dormancy in Aspergillus fumigatus, two dehydrin-like genes, DprA and DprB, were identified. The deduced proteins had repeated stretches of 23 amino acids that contained a conserved dehydrin-like protein (DPR) motif. Disrupted DprAΔ mutants were hypersensitive to oxidative stress and to phagocytic killing, whereas DprBΔ mutants were impaired in osmotic and pH stress responses. However, no effect was observed on their pathogenicity in our experimental models of invasive aspergillosis. Molecular dissection of the signaling pathways acting upstream showed that expression of DprA was dependent on the stress-activated kinase SakA and the cyclic AMP-protein kinase A (cAMP-PKA) pathways, which activate the bZIP transcription factor AtfA, while expression of DprB was dependent on the SakA mitogen-activated protein kinase (MAPK) pathway, and the zinc finger transcription factor PacC. Fluorescent protein fusions showed that both proteins were associated with peroxisomes and the cytosol. Accordingly, DprA and DprB were important for peroxisome function. Our findings reveal a novel family of stress-protective proteins in A. fumigatus and, potentially, in filamentous ascomycetes.
Pubmed
Web of science
Open Access
Yes
Create date
14/06/2011 13:05
Last modification date
20/08/2019 14:20
Usage data