Analysis of a gene that suppresses the morphological defect of bald mutants of Streptomyces griseus.

Details

Serval ID
serval:BIB_635BA9D7D09F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Analysis of a gene that suppresses the morphological defect of bald mutants of Streptomyces griseus.
Journal
Journal of Bacteriology
Author(s)
McCue L.A., Kwak J., Wang J., Kendrick K.E.
ISSN
0021-9193
Publication state
Published
Issued date
1996
Peer-reviewed
Oui
Volume
178
Number
10
Pages
2867-2875
Language
english
Abstract
When present in multiple copies, orf1590 restored sporulation to class IIIA bald mutants of Streptomyces griseus, which form sporulation septa and thick spore walls prematurely. The orf1590 alleles from class IIIA bald mutants restored sporulation upon introduction at a high copy number into those same mutants, and the nucleotide sequence of one of these alleles was identical to that of the wild-type strain. We conclude that overexpression of orf1590 suppresses the defect in class IIIA bald mutants. Previous nucleotide sequence and transcript analyses suggested that orf1590 could encode two related proteins, P56 and P49.5, from nested coding sequences. A mutation that prevented the synthesis of P56 without altering the coding sequence for P49.5 eliminated the function of orf1590, as did amino acid substitutions in the putative helix-turn-helix domain located at the N terminus of P56 and absent from P49.5. To determine the coding capacity of orf1590, we analyzed translational fusions between orf1590 and the neo gene from Tn5. Measurement of the expression of fusions to the wild-type and mutant alleles of orf1590 indicated that P56 was the sole product of orf1590 during vegetative growth. Attempts to generate a nonfunctional frameshift mutation in orf1590 were unsuccessful in the absence of a second-site bald mutation, suggesting that orf1590 may be required during vegetative growth by preventing early sporulation. Our results are consistent with the hypothesis that P56 at a high level delays the premature synthesis of sporulation septa and spore walls in class IIIA mutants.
Keywords
Amino Acid Sequence, Base Sequence, Genes, Bacterial, Models, Genetic, Molecular Sequence Data, Morphogenesis/genetics, Mutation, Protein Biosynthesis, Protein Structure, Secondary, RNA, Bacterial/genetics, RNA, Messenger/genetics, Sequence Analysis, DNA, Spores, Bacterial, Streptomyces griseus/cytology, Streptomyces griseus/genetics, Suppression, Genetic, Transcription, Genetic, Transformation, Genetic
Pubmed
Web of science
Create date
19/11/2007 10:30
Last modification date
20/08/2019 14:19
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