Identification of a new murine tumor necrosis factor receptor locus that contains two novel murine receptors for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL).

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Serval ID
serval:BIB_6342D5564CA3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Identification of a new murine tumor necrosis factor receptor locus that contains two novel murine receptors for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL).
Journal
Journal of Biological Chemistry
Author(s)
Schneider P., Olson D., Tardivel A., Browning B., Lugovskoy A., Gong D., Dobles M., Hertig S., Hofmann K., Van Vlijmen H., Hsu Y.M., Burkly L.C., Tschopp J., Zheng T.S.
ISSN
0021-9258 (Print)
ISSN-L
0021-9258
Publication state
Published
Issued date
2003
Volume
278
Number
7
Pages
5444-5454
Language
english
Abstract
Tumor necrosis factor (TNF) ligand and receptor superfamily members play critical roles in diverse developmental and pathological settings. In search for novel TNF superfamily members, we identified a murine chromosomal locus that contains three new TNF receptor-related genes. Sequence alignments suggest that the ligand binding regions of these murine TNF receptor homologues, mTNFRH1, -2 and -3, are most homologous to those of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptors. By using a number of in vitro ligand-receptor binding assays, we demonstrate that mTNFRH1 and -2, but not mTNFRH3, bind murine TRAIL, suggesting that they are indeed TRAIL receptors. This notion is further supported by our demonstration that both mTNFRH1:Fc and mTNFRH2:Fc fusion proteins inhibited mTRAIL-induced apoptosis of Jurkat cells. Unlike the only other known murine TRAIL receptor mTRAILR2, however, neither mTNFRH2 nor mTNFRH3 has a cytoplasmic region containing the well characterized death domain motif. Coupled with our observation that overexpression of mTNFRH1 and -2 in 293T cells neither induces apoptosis nor triggers NFkappaB activation, we propose that the mTnfrh1 and mTnfrh2 genes encode the first described murine decoy receptors for TRAIL, and we renamed them mDcTrailr1 and -r2, respectively. Interestingly, the overall sequence structures of mDcTRAILR1 and -R2 are quite distinct from those of the known human decoy TRAIL receptors, suggesting that the presence of TRAIL decoy receptors represents a more recent evolutionary event.
Keywords
Amino Acid Sequence, Animals, Apoptosis Regulatory Proteins, Cell Line, Chromosome Mapping, Evolution, Molecular, Humans, Membrane Glycoproteins/genetics, Membrane Glycoproteins/metabolism, Mice, Models, Molecular, Molecular Sequence Data, Receptors, Tumor Necrosis Factor/genetics, Receptors, Tumor Necrosis Factor/metabolism, Sequence Analysis, TNF-Related Apoptosis-Inducing Ligand, Tumor Necrosis Factor-alpha/genetics, Tumor Necrosis Factor-alpha/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:18
Last modification date
20/08/2019 14:19
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