Investigating the Effect of Aloe vera Gel on the Buccal Permeability of Didanosine.

Details

Serval ID
serval:BIB_6331156E2F83
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Investigating the Effect of Aloe vera Gel on the Buccal Permeability of Didanosine.
Journal
Planta Medica
Author(s)
Ojewole E., Mackraj I., Akhundov K., Hamman J., Viljoen A., Olivier E., Wesley-Smith J., Govender T.
ISSN
1439-0221 (Electronic)
ISSN-L
0032-0943
Publication state
Published
Issued date
2012
Volume
78
Number
4
Pages
354-361
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
The buccal mucosal route offers several advantages but the delivery of certain drugs can be limited by low membrane permeability. This study investigated the buccal permeability properties of didanosine (ddI) and assessed the potential of ALOE VERA gel (AVgel) as a novel buccal permeation enhancer. Permeation studies were performed using Franz diffusion cells, and the drug was quantified by UV spectroscopy. Histomorphological evaluations were undertaken using light and transmission electron microscopy. The permeability of ddI was concentration-dependent, and it did not have any adverse effects on the buccal mucosae. A linear relationship (R (2) = 0.9557) between the concentrations and flux indicated passive diffusion as the mechanism of drug transport. AVgel at concentrations of 0.25 to 2 %w/v enhanced ddI permeability with enhancement ratios from 5.09 (0.25 %w/v) to 11.78 (2 %w/v) but decreased permeability at 4 and 6 %w/v. Ultrastructural analysis of the buccal mucosae treated with phosphate buffer saline pH 7.4 (PBS), ddI/PBS, and ddI/PBS/AVgel 0.5 %w/v showed cells with normal plasmalemma, well-developed cristae, and nuclei with regular nuclear envelopes. However, cells from 1, 2, and 6 %w/v AVgel-treated mucosae showed irregular nuclear outlines, increased intercellular spacing, and plasmalemma crenulations. This study demonstrates the potential of AVgel as a buccal permeation enhancer for ddI to improve anti-HIV and AIDS therapy.
Pubmed
Web of science
Create date
01/04/2012 13:59
Last modification date
20/08/2019 14:19
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