Strain diversity of mycobacteria isolated from pulmonary tuberculosis patients at Debre Birhan Hospital, Ethiopia.

Details

Serval ID
serval:BIB_632691A97ABE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Strain diversity of mycobacteria isolated from pulmonary tuberculosis patients at Debre Birhan Hospital, Ethiopia.
Journal
International Journal of Tuberculosis and Lung Disease
Author(s)
Garedew L., Mihret A., Mamo G., Abebe T., Firdessa R., Bekele Y., Ameni G.
ISSN
1815-7920 (Electronic)
ISSN-L
1027-3719
Publication state
Published
Issued date
2013
Volume
17
Number
8
Pages
1076-1081
Language
english
Abstract
BACKGROUND: Ethiopia ranks seventh in the list of 22 high tuberculosis (TB) burden countries, with an incidence rate of 379 cases per 100 000 population for TB all forms. However, information on the genomic diversity of Mycobacterium tuberculosis in Ethiopia is limited.
OBJECTIVE: To investigate the molecular characteristics of M. tuberculosis strains implicated in pulmonary TB in the study area.
METHODS AND RESULTS: A cross-sectional study was conducted using socio-demographic, clinical and culture data combined with molecular typing analysis. The proportion of TB and M. tuberculosis isolates was not associated with risk factors (P > 0.05). Of 99 sputum samples, 80.8% were culture-positive. Speciation of isolates showed that 88.8% were M. tuberculosis. Further characterisation led to the identification of 27 different spoligotype patterns of M. tuberculosis; the most dominant shared types were SIT149, SIT53 and SIT54. Of the 27 strains, three strains were new and were reported to the SITVIT database. More than two thirds of the strains belonged to the Euro-American lineage.
CONCLUSION: This study shows the presence of several clusters and new strains of M. tuberculosis circulating in pulmonary TB patients in the study area, suggesting recent transmission. Nationwide studies are recommended to map the population structure of M. tuberculosis and set control measures.
Keywords
pulmonary tuberculosis, sputum, molecular typing, cluster formation, recent transmission
Pubmed
Web of science
Create date
22/08/2013 7:43
Last modification date
20/08/2019 14:19
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