Recording from Fly Olfactory Sensilla.

Details

Serval ID
serval:BIB_63261D3B78B2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Recording from Fly Olfactory Sensilla.
Journal
Cold Spring Harbor protocols
Author(s)
Benton R., Dahanukar A.
ISSN
1559-6095 (Electronic)
ISSN-L
1559-6095
Publication state
Published
Issued date
03/04/2023
Peer-reviewed
Oui
Volume
2023
Number
4
Pages
pdb.prot108063
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Olfactory systems detect and discriminate an enormous diversity of volatile environmental stimuli and provide important paradigms to investigate how sensory cues are represented in the brain. Key stimulus-coding events occur in peripheral olfactory sensory neurons, which typically express a single olfactory receptor-from a large repertoire encoded in the genome-with a defined ligand-response profile. These receptors convert odor ligand recognition into spatial and temporal patterns of neural activity that are transmitted to, and interpreted in, central brain regions. Drosophila provides an attractive model to study olfactory coding because it possesses a relatively simple peripheral olfactory system that displays many organizational parallels to those of vertebrates. Moreover, nearly all olfactory sensory neurons have been molecularly characterized and are accessible for physiological analysis, as they are exposed on the surface of sensory organs (antennae and maxillary palps) housed in specialized hairs called sensilla. This protocol describes how to perform recordings of odor-evoked activity from Drosophila olfactory sensilla, covering the basics of sample preparation, setting up the electrophysiology rig, assembling an odor stimulus-delivery device, and data analysis. The methodology can be used to characterize the ligand-recognition properties of most olfactory sensory neurons and the role of olfactory receptors (and other molecular components) in signal transduction.
Keywords
Animals, Sensilla/physiology, Ligands, Olfactory Receptor Neurons/physiology, Smell/physiology, Odorants, Drosophila
Pubmed
Create date
30/11/2022 10:06
Last modification date
12/04/2023 6:54
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