Fast Diagnostics of BRAF Mutations in Biopsies from Malignant Melanoma.

Details

Serval ID
serval:BIB_63156C14F0E9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Fast Diagnostics of BRAF Mutations in Biopsies from Malignant Melanoma.
Journal
Nano letters
Author(s)
Huber F., Lang H.P., Glatz K., Rimoldi D., Meyer E., Gerber C.
ISSN
1530-6992 (Electronic)
ISSN-L
1530-6984
Publication state
Published
Issued date
14/09/2016
Peer-reviewed
Oui
Volume
16
Number
9
Pages
5373-5377
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
According to the American skin cancer foundation, there are more new cases of skin cancer than the combined incidence of cancers of the breast, prostate, lung, and colon each year, and malignant melanoma represents its deadliest form. About 50% of all cases are characterized by a particular mutation BRAF(V600E) in the BRAF (Rapid Acceleration of Fibrosarcoma gene B) gene. Recently developed highly specific drugs are able to fight BRAF(V600E) mutated tumors but require diagnostic tools for fast and reliable mutation detection to warrant treatment efficiency. We completed a preliminary clinical trial applying cantilever array sensors to demonstrate identification of a BRAF(V600E) single-point mutation using total RNA obtained from biopsies of metastatic melanoma of diverse sources (surgical material either frozen or fixated with formalin and embedded in paraffin). The method is faster than the standard Sanger or pyrosequencing methods and comparably sensitive as next-generation sequencing. Processing time from biopsy to diagnosis is below 1 day and does not require PCR amplification, sequencing, and labels.
Keywords
Biopsy, DNA Mutational Analysis, Humans, Melanoma/genetics, Mutation, Proto-Oncogene Proteins B-raf/genetics, Skin Neoplasms/genetics, Nanomechanical microcantilever, biosensor, malignant melanoma, skin cancer
Pubmed
Web of science
Create date
12/08/2016 10:44
Last modification date
21/08/2019 5:15
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