Transcriptional and functional profiles of voltage-gated Na(+) channels in injured and non-injured DRG neurons in the SNI model of neuropathic pain.

Details

Serval ID
serval:BIB_62A649E6A2E8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Transcriptional and functional profiles of voltage-gated Na(+) channels in injured and non-injured DRG neurons in the SNI model of neuropathic pain.
Journal
Molecular and Cellular Neuroscience
Author(s)
Berta T., Poirot O., Pertin M., Ji R.R., Kellenberger S., Decosterd I.
ISSN
1044-7431
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
37
Number
2
Pages
196-208
Language
english
Abstract
Changes in expression and function of voltage-gated sodium channels (VGSC) in dorsal root ganglion (DRG) neurons may play a major role in the genesis of peripheral hyperexcitability that occurs in neuropathic pain. We present here the first description of changes induced by spared nerve injury (SNI) to Na(v)1 mRNA levels and tetrodotoxin-sensitive and -resistant (TTX-S/TTX-R) Na(+) currents in injured and adjacent non-injured small DRG neurons. VGSC transcripts were down-regulated in injured neurons except for Na(v)1.3, which increased, while they were either unchanged or increased in non-injured neurons. TTX-R current densities were reduced in injured neurons and the voltage dependence of steady-state inactivation for TTX-R was positively shifted in injured and non-injured neurons. TTX-S current densities were not affected by SNI, while the rate of recovery from inactivation was accelerated in injured neurons. Our results describe altered neuronal electrogenesis following SNI that is likely induced by a complex regulation of VGSCs.
Keywords
Animals, Cells, Cultured, Disease Models, Animal, Down-Regulation/genetics, Ganglia, Spinal/injuries, Ganglia, Spinal/metabolism, Gene Expression Regulation/genetics, Ion Channel Gating/genetics, Male, Membrane Potentials/genetics, Nerve Tissue Proteins/genetics, Neuralgia/genetics, Neuralgia/metabolism, Nociceptors/metabolism, Nociceptors/physiopathology, Peripheral Nervous System Diseases/genetics, Peripheral Nervous System Diseases/metabolism, Protein Subunits/genetics, RNA, Messenger/metabolism, Rats, Recovery of Function/genetics, Sodium Channel Blockers/pharmacology, Sodium Channels/genetics
Pubmed
Web of science
Create date
29/01/2009 22:13
Last modification date
20/08/2019 14:19
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