Forensic identification of urine samples: a comparison between nuclear and mitochondrial DNA markers.

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Serval ID
serval:BIB_629B48BFB294
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Forensic identification of urine samples: a comparison between nuclear and mitochondrial DNA markers.
Journal
International journal of legal medicine
Author(s)
Castella V., Dimo-Simonin N., Brandt-Casadevall C., Robinson N., Saugy M., Taroni F., Mangin P.
ISSN
0937-9827
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
120
Number
2
Pages
67-72
Language
english
Notes
Publication types: Journal Article - Publication Status: ppublish
Abstract
Urine samples from 20 male volunteers of European Caucasian origin were stored at 4 degrees C over a 4-month period in order to compare the identification potential of nuclear DNA (nDNA) and mitochondrial DNA (mtDNA) markers. The amount of nDNA recovered from urines dramatically declined over time. Consequently, nDNA likelihood ratios (LRs) greater than 1,000 were obtained for 100, 70 and 55% of the urines analysed after 6, 60 and 120 days, respectively. For the mtDNA, HVI and HVII sequences were obtained for all samples tested, whatever the period considered. Nevertheless, the highest mtDNA LR of 435 was relatively low compared to its nDNA equivalent. Indeed, LRs obtained with only three nDNA loci could easily exceed this value and are quite easier to obtain. Overall, the joint use of nDNA and mtDNA markers enabled the 20 urine samples to be identified, even after the 4-month period.
Keywords
Cell Nucleus, Complementarity Determining Regions, DNA, Mitochondrial, European Continental Ancestry Group, Forensic Genetics, Genetic Markers, Humans, Likelihood Functions, Male, Polymerase Chain Reaction, Sequence Analysis, DNA, Specimen Handling, Time Factors
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 11:30
Last modification date
01/10/2019 7:18
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