The dermatophyte species Arthroderma benhamiae: intraspecies variability and mating behaviour.

Details

Serval ID
serval:BIB_629512E0E826
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The dermatophyte species Arthroderma benhamiae: intraspecies variability and mating behaviour.
Journal
Journal of Medical Microbiology
Author(s)
Symoens F., Jousson O., Packeu A., Fratti M., Staib P., Mignon B., Monod M.
ISSN
1473-5644 (Electronic)
ISSN-L
0022-2615
Publication state
Published
Issued date
2013
Volume
62
Number
Pt 3
Pages
377-385
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
Arthroderma benhamiae is a zoophilic dermatophyte belonging to the Trichophyton mentagrophytes species complex. Here, a population of A. benhamiae wild strains from the same geographical area (Switzerland) was studied by comparing their morphology, assessing their molecular variability using internal transcribed spacer (ITS) and 28S rRNA gene sequencing, and evaluating their interfertility. Sequencing of the ITS region and of part of the 28S rRNA gene revealed the existence of two infraspecific groups with markedly different colony phenotypes: white (group I) and yellow (group II), respectively. For all strains, the results of mating type identification by PCR, using HMG (high-mobility group) and α-box genes in the mating type locus as targets, were in total accordance with the results of mating type identification by strain confrontation experiments. White-phenotype strains were of mating type + (mt+) or mating type - (mt-), whilst yellow-phenotype strains were all mt-. White and yellow strains were found to produce fertile cleistothecia after mating with A. benhamiae reference tester strains, which belonged to a third group intermediate between groups I and II. However, no interfertility was observed between yellow strains and white strains of mt+. A significant result was that white strains of mt- were able to mate and produce fertile cleistothecia with the white A. benhamiae strain CBS 112371 (mt+), the genome of which has recently been sequenced and annotated. This finding should offer new tools for investigating the biology and genetics of dermatophytes using wild-type strains.
Keywords
Animals, Arthrodermataceae/classification, Arthrodermataceae/genetics, DNA, Fungal/chemistry, DNA, Fungal/genetics, DNA, Ribosomal Spacer/genetics, Dogs, Fungal Proteins/genetics, Fungal Proteins/metabolism, Gene Expression Regulation, Fungal, Genes, Mating Type, Fungal, Guinea Pigs, Humans, Molecular Sequence Data, RNA, Ribosomal, 28S/genetics, Tinea/microbiology
Pubmed
Web of science
Create date
21/04/2013 9:35
Last modification date
20/08/2019 15:19
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