The role of antibody concentration and avidity in antiviral protection.

Details

Serval ID
serval:BIB_62796F395CA7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The role of antibody concentration and avidity in antiviral protection.
Journal
Science
Author(s)
Bachmann M.F., Kalinke U., Althage A., Freer G., Burkhart C., Roost H., Aguet M., Hengartner H., Zinkernagel R.M.
ISSN
0036-8075 (Print)
ISSN-L
0036-8075
Publication state
Published
Issued date
1997
Volume
276
Number
5321
Pages
2024-2027
Language
english
Abstract
Neutralizing antibodies are necessary and sufficient for protection against infection with vesicular stomatitis virus (VSV). The in vitro neutralization capacities and in vivo protective capacities of a panel of immunoglobulin G monoclonal antibodies to the glycoprotein of VSV were evaluated. In vitro, neutralizing activity correlated with avidity and with neutralization rate constant, a measure of on-rate. However, in vivo, protection was independent of immunoglobulin subclass, avidity, neutralization rate constant, and in vitro neutralizing activity; above a minimal avidity threshold, protection depended simply on a minimum serum concentration. These two biologically defined thresholds of antibody specificity offer hope for the development of adoptive therapy with neutralizing antibodies.
Keywords
Animals, Antibodies, Monoclonal/blood, Antibodies, Monoclonal/immunology, Antibodies, Viral/blood, Antibodies, Viral/immunology, Antibody Affinity, Antibody Specificity, Brain/virology, Enzyme-Linked Immunosorbent Assay, Glycoproteins/immunology, Immunization, Passive, Immunoglobulin G/blood, Immunoglobulin G/immunology, Membrane Glycoproteins, Mice, Mice, Inbred BALB C, Mice, SCID, Neutralization Tests, Rhabdoviridae Infections/immunology, Rhabdoviridae Infections/virology, Vesicular stomatitis Indiana virus/growth & development, Vesicular stomatitis Indiana virus/immunology, Viral Envelope Proteins/immunology
Pubmed
Web of science
Create date
28/01/2008 12:36
Last modification date
20/08/2019 15:19
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