The role of antibody concentration and avidity in antiviral protection.
Details
Serval ID
serval:BIB_62796F395CA7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The role of antibody concentration and avidity in antiviral protection.
Journal
Science
ISSN
0036-8075 (Print)
ISSN-L
0036-8075
Publication state
Published
Issued date
1997
Volume
276
Number
5321
Pages
2024-2027
Language
english
Abstract
Neutralizing antibodies are necessary and sufficient for protection against infection with vesicular stomatitis virus (VSV). The in vitro neutralization capacities and in vivo protective capacities of a panel of immunoglobulin G monoclonal antibodies to the glycoprotein of VSV were evaluated. In vitro, neutralizing activity correlated with avidity and with neutralization rate constant, a measure of on-rate. However, in vivo, protection was independent of immunoglobulin subclass, avidity, neutralization rate constant, and in vitro neutralizing activity; above a minimal avidity threshold, protection depended simply on a minimum serum concentration. These two biologically defined thresholds of antibody specificity offer hope for the development of adoptive therapy with neutralizing antibodies.
Keywords
Animals, Antibodies, Monoclonal/blood, Antibodies, Monoclonal/immunology, Antibodies, Viral/blood, Antibodies, Viral/immunology, Antibody Affinity, Antibody Specificity, Brain/virology, Enzyme-Linked Immunosorbent Assay, Glycoproteins/immunology, Immunization, Passive, Immunoglobulin G/blood, Immunoglobulin G/immunology, Membrane Glycoproteins, Mice, Mice, Inbred BALB C, Mice, SCID, Neutralization Tests, Rhabdoviridae Infections/immunology, Rhabdoviridae Infections/virology, Vesicular stomatitis Indiana virus/growth & development, Vesicular stomatitis Indiana virus/immunology, Viral Envelope Proteins/immunology
Pubmed
Web of science
Create date
28/01/2008 12:36
Last modification date
20/08/2019 15:19