Functional architecture of olfactory ionotropic glutamate receptors.

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State: Public
Version: Author's accepted manuscript
Serval ID
serval:BIB_62747B9FEB54
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Functional architecture of olfactory ionotropic glutamate receptors.
Journal
Neuron
Author(s)
Abuin L., Bargeton B., Ulbrich M.H., Isacoff E.Y., Kellenberger S., Benton R.
ISSN
1097-4199 (Electronic)
ISSN-L
0896-6273
Publication state
Published
Issued date
2011
Volume
69
Number
1
Pages
44-60
Language
english
Abstract
Ionotropic glutamate receptors (iGluRs) are ligand-gated ion channels that mediate chemical communication between neurons at synapses. A variant iGluR subfamily, the Ionotropic Receptors (IRs), was recently proposed to detect environmental volatile chemicals in olfactory cilia. Here, we elucidate how these peripheral chemosensors have evolved mechanistically from their iGluR ancestors. Using a Drosophila model, we demonstrate that IRs act in combinations of up to three subunits, comprising individual odor-specific receptors and one or two broadly expressed coreceptors. Heteromeric IR complex formation is necessary and sufficient for trafficking to cilia and mediating odor-evoked electrophysiological responses in vivo and in vitro. IRs display heterogeneous ion conduction specificities related to their variable pore sequences, and divergent ligand-binding domains function in odor recognition and cilia localization. Our results provide insights into the conserved and distinct architecture of these olfactory and synaptic ion channels and offer perspectives into the use of IRs as genetically encoded chemical sensors. VIDEO ABSTRACT:
Keywords
Animals, Cilia/physiology, Drosophila, Drosophila Proteins/metabolism, Electrophysiology, Evoked Potentials/physiology, Fluorescent Antibody Technique, Odors, Olfactory Bulb/cytology, Olfactory Bulb/physiology, Olfactory Receptor Neurons/metabolism, Olfactory Receptor Neurons/physiology, Receptors, Ionotropic Glutamate/metabolism, Receptors, Odorant/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
22/03/2011 15:53
Last modification date
20/10/2020 10:12
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