Loss-of-function of the long non-coding RNA A830019P07Rik in mice does not affect insulin expression and secretion.

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Ressource 1Download: Guay Sicentific Reports 2020 P07Rik KO mice.pdf (1220.05 [Ko])
State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_6245EFD1FD07
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Loss-of-function of the long non-coding RNA A830019P07Rik in mice does not affect insulin expression and secretion.
Journal
Scientific reports
Author(s)
Guay C., Abdulkarim B., Tan J.Y., Dubuis G., Rütti S., Ross Laybutt D., Widmann C., Regazzi R., Marques A.C.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
14/04/2020
Peer-reviewed
Oui
Volume
10
Number
1
Pages
6413
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Long non-coding RNAs (lncRNAs) contribute to diverse cellular functions and the dysregulation of their expression or function can contribute to diseases, including diabetes. The contributions of lncRNAs to β-cell development, function and survival has been extensively studied in vitro. However, very little is currently known on the in vivo roles of lncRNAs in the regulation of glucose and insulin homeostasis. Here we investigated the impact of loss-of-function in mice of the lncRNA A830019P07Rik, hereafter P07Rik, which was previously reported to be associated with reduced plasma insulin levels. Compared with wild-type littermates, male and female P07Rik mutant mice did not show any defect in glycaemia and plasma insulin levels in both fed and fasted state. Furthermore, P07Rik mutant mice displayed similar glucose and insulin levels in response to an intra-peritoneal glucose tolerance test. Ex vivo, islets from mutant P07Rik released similar amount of insulin in response to increased glucose concentration as wildtype littermates. In contrast with previous reports, our characterization of P07Rik mouse mutants revealed that loss of function of this lncRNA does not affect glucose and insulin homeostasis in mice.
Keywords
lncRNA, P07Rik, glucose homeostasis, beta-cell function, islets, mice
Pubmed
Open Access
Yes
Create date
25/04/2020 20:57
Last modification date
22/07/2020 7:09
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