The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress
Details
Serval ID
serval:BIB_622708F76276
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress
Journal
Science
ISSN
1095-9203 (Electronic)
Publication state
Published
Issued date
05/2004
Volume
304
Number
5672
Pages
843-6
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May 7
Research Support, Non-U.S. Gov't --- Old month value: May 7
Abstract
Apoptosis is triggered by activation of initiator caspases upon complex-mediated clustering of the inactive zymogen, as occurs in the caspase-9-activating apoptosome complex. Likewise, caspase-2, which is involved in stress-induced apoptosis, is recruited into a large protein complex, the molecular composition of which remains elusive. We show that activation of caspase-2 occurs in a complex that contains the death domain-containing protein PIDD, whose expression is induced by p53, and the adaptor protein RAIDD. Increased PIDD expression resulted in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli. Because PIDD functions in p53-mediated apoptosis, the complex assembled by PIDD and caspase-2 is likely to regulate apoptosis induced by genotoxins.
Keywords
*Adaptor Proteins, Signal Transducing
*Apoptosis
CRADD Signaling Adaptor Protein
Carrier Proteins/chemistry/*metabolism
Caspase 2
Caspases/*metabolism
Cell Line
Cell Line, Tumor
Cloning, Molecular
*DNA Damage
Doxorubicin/pharmacology
Enzyme Activation
Etoposide/pharmacology
Humans
Protein Structure, Tertiary
Proteins/chemistry/metabolism
RNA, Small Interfering
Signal Transduction
Transfection
Tumor Suppressor Protein p53/metabolism
Pubmed
Web of science
Create date
24/01/2008 15:19
Last modification date
20/08/2019 14:19