A clinal polymorphism in the insulin signaling transcription factor foxo contributes to life-history adaptation in Drosophila.

Details

Serval ID
serval:BIB_622579E5505B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A clinal polymorphism in the insulin signaling transcription factor foxo contributes to life-history adaptation in Drosophila.
Journal
Evolution; international journal of organic evolution
Author(s)
Durmaz E., Rajpurohit S., Betancourt N., Fabian D.K., Kapun M., Schmidt P., Flatt T.
ISSN
1558-5646 (Electronic)
ISSN-L
0014-3820
Publication state
Published
Issued date
09/2019
Peer-reviewed
Oui
Volume
73
Number
9
Pages
1774-1792
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
A fundamental aim of adaptation genomics is to identify polymorphisms that underpin variation in fitness traits. In Drosophila melanogaster, latitudinal life-history clines exist on multiple continents and make an excellent system for dissecting the genetics of adaptation. We have previously identified numerous clinal single-nucleotide polymorphism in insulin/insulin-like growth factor signaling (IIS), a pathway known from mutant studies to affect life history. However, the effects of natural variants in this pathway remain poorly understood. Here we investigate how two clinal alternative alleles at foxo, a transcriptional effector of IIS, affect fitness components (viability, size, starvation resistance, fat content). We assessed this polymorphism from the North American cline by reconstituting outbred populations, fixed for either the low- or high-latitude allele, from inbred DGRP lines. Because diet and temperature modulate IIS, we phenotyped alleles across two temperatures (18°C, 25°C) and two diets differing in sugar source and content. Consistent with clinal expectations, the high-latitude allele conferred larger body size and reduced wing loading. Alleles also differed in starvation resistance and expression of insulin-like receptor, a transcriptional target of FOXO. Allelic reaction norms were mostly parallel, with few GxE interactions. Together, our results suggest that variation in IIS makes a major contribution to clinal life-history adaptation.
Keywords
Adaptation, Physiological, Alleles, Animals, Body Size, Drosophila Proteins/genetics, Drosophila Proteins/physiology, Drosophila melanogaster/genetics, Drosophila melanogaster/physiology, Female, Forkhead Transcription Factors/genetics, Forkhead Transcription Factors/physiology, Genetic Variation, Genetics, Population, Genomics, Genotype, Insulin/metabolism, Male, Mutation, Phenotype, Polymorphism, Single Nucleotide, Signal Transduction, Temperature, Wings, Animal, Adaptation, cline, insulin signaling, life history, plasticity, pleiotropy
Pubmed
Web of science
Open Access
Yes
Create date
25/05/2019 10:37
Last modification date
29/08/2020 5:20
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