Increased numbers of circulating polyfunctional Th17 memory cells in patients with seronegative spondylarthritides

Details

Serval ID
serval:BIB_620816C7D716
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Increased numbers of circulating polyfunctional Th17 memory cells in patients with seronegative spondylarthritides
Journal
Arthritis and Rheumatism
Author(s)
Jandus C., Bioley G., Rivals J.P., Dudler J., Speiser D., Romero P.
ISSN
0004-3591
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
58
Number
8
Pages
2307-2317
Language
english
Abstract
OBJECTIVE: A distinct subset of proinflammatory CD4+ T cells that produce interleukin-17 was recently identified. These cells are implicated in different autoimmune disease models, such as experimental autoimmune encephalomyelitis and collagen-induced arthritis, but their involvement in human autoimmune disease has not yet been clearly established. The purpose of this study was to assess the frequency and functional properties of Th17 cells in healthy donors and in patients with different autoimmune diseases. METHODS: Peripheral blood was obtained from 10 psoriatic arthritis (PsA), 10 ankylosing spondylitis (AS), 10 rheumatoid arthritis (RA), and 5 vitiligo patients, as well as from 25 healthy donors. Synovial tissue samples from a separate group of patients were also evaluated (obtained as paraffin-embedded sections). Peripheral blood cells were analyzed by multiparameter flow cytometry and immunohistochemistry. Cytokine production was examined by enzyme-linked immunosorbent assay and intracellular cytokine staining using specific monoclonal antibodies. Synovial tissue was examined for infiltrating T cells by immunohistochemical analysis. RESULTS: We found increased numbers of circulating Th17 cells in the peripheral blood of patients with seronegative spondylarthritides (PsA and AS), but not in patients with RA or vitiligo. In addition, Th17 cells from the spondylarthritis patients showed advanced differentiation and were polyfunctional in terms of T cell receptor-driven cytokine production. CONCLUSION: These observations suggest a role of Th17 cells in the pathogenesis of certain human autoimmune disorders, in particular the seronegative spondylarthritides.
Keywords
Arthritis, Psoriatic, Arthritis, Rheumatoid, CD4-Positive T-Lymphocytes, Case-Control Studies, Cell Differentiation, Humans, Interleukin-17, Leukocytes, Mononuclear, Receptors, CCR6, Spondylarthritis, Spondylitis, Ankylosing, T-Lymphocyte Subsets, T-Lymphocytes, Regulatory, Vitiligo
Pubmed
Web of science
Open Access
Yes
Create date
22/01/2009 12:47
Last modification date
20/08/2019 14:19
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