Presentation of T-cell epitopes assembled as multiple-antigen peptides to murine and human T lymphocytes

Details

Serval ID
serval:BIB_61F3D5725757
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Presentation of T-cell epitopes assembled as multiple-antigen peptides to murine and human T lymphocytes
Journal
Infection and Immunity
Author(s)
Grillot  D., Valmori  D., Lambert  P. H., Corradin  G., Del Giudice  G.
ISSN
0019-9567 (Print)
Publication state
Published
Issued date
07/1993
Volume
61
Number
7
Pages
3064-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Abstract
Multiple-antigen peptide (MAP) constructs containing different T- and B-cell epitopes were assessed for their ability to be specifically recognized by murine and human T-cell clones. The different synthetic MAP constructs consisted of a malaria T-cell epitope or of a human universal tetanus toxin helper T-cell epitope collinearly synthesized with B-cell epitopes from the circumsporozoite proteins of different malaria parasites. All constructs were able to stimulate specifically T-cell clones. Interestingly, T-cell epitopes assembled as MAP constructs did not require processing for the specific stimulation of murine and human T-cell clones, as shown by retention of their stimulatory effect in the presence of glutaraldehyde-fixed antigen-presenting cells. However, processing was required for most of the synthetic constructs containing both T- and B-cell epitopes. Thus, the requirement for processing of these constructs seems to be dictated by the nature of the B-cell epitope present.
Keywords
Amino Acid Sequence Animals Antigen-Presenting Cells/*immunology *Epitopes Humans Mice Mice, Inbred BALB C Mice, Inbred C57BL Molecular Sequence Data Plasmodium yoelii/immunology Protozoan Proteins/immunology T-Lymphocytes/*immunology
Pubmed
Web of science
Create date
24/01/2008 15:55
Last modification date
20/08/2019 15:18
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