Localization and fine structure of a vaccinia virus gene encoding an envelope antigen.

Details

Serval ID
serval:BIB_61CD51391BE3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Localization and fine structure of a vaccinia virus gene encoding an envelope antigen.
Journal
Journal of Virology
Author(s)
Hirt P., Hiller G., Wittek R.
ISSN
0022-538X[print], 0022-538X[linking]
Publication state
Published
Issued date
06/1986
Volume
58
Number
3
Pages
757-764
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The major antigen on the envelope of extracellular vaccinia virus particles is a polypeptide with an apparent molecular weight of 37,000 (p37K; G. Hiller and K. Weber, J. Virol. 55:651-659, 1985). The gene encoding p37K was mapped in the vaccinia virus genome by hybrid selection of RNA followed by in vitro translation. p37K was then identified among the in vitro translation products by immunoprecipitation with a monoclonal antibody. The gene is located close to the right-hand end of the HindIII F fragment. The corresponding region of the DNA was sequenced, and an open reading frame encoding a polypeptide of 41,748 daltons was observed. The 5' end of the mRNA, as defined by nuclease S1 analysis, maps within only a few nucleotides of the translation initiation codon. Examination of the DNA sequence around the putative initiation site of transcription revealed a characteristic sequence, TAAATG, which includes the ATG translation initiation codon and which is conserved in all but one late gene so far analyzed. It is therefore likely that this sequence is an important regulatory signal for late gene expression in vaccinia virus.
Keywords
Amino Acid Sequence, Antigens, Viral/analysis, Antigens, Viral/genetics, Base Sequence, Chemical Precipitation, Chromosome Mapping, DNA, Viral/analysis, Genes, Viral, RNA, Messenger/analysis, Vaccinia virus/genetics, Viral Envelope Proteins/analysis, Viral Envelope Proteins/genetics, Virus Replication
Pubmed
Web of science
Create date
24/01/2008 10:43
Last modification date
20/08/2019 14:18
Usage data