Small animal magnetic resonance imaging: an efficient tool to assess liver volume and intrahepatic vascular anatomy.

Details

Serval ID
serval:BIB_61AE4F5943C8
Type
Article: article from journal or magazin.
Collection
Publications
Title
Small animal magnetic resonance imaging: an efficient tool to assess liver volume and intrahepatic vascular anatomy.
Journal
The Journal of surgical research
Author(s)
Melloul E., Raptis D.A., Boss A., Pfammater T., Tschuor C., Tian Y., Graf R., Clavien P.A., Lesurtel M.
ISSN
1095-8673 (Electronic)
ISSN-L
0022-4804
Publication state
Published
Issued date
04/2014
Volume
187
Number
2
Pages
458-465
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
To develop a noninvasive technique to assess liver volumetry and intrahepatic portal vein anatomy in a mouse model of liver regeneration.
Fifty-two C57BL/6 male mice underwent magnetic resonance imaging (MRI) of the liver using a 4.7 T small animal MRI system after no treatment, 70% partial hepatectomy (PH), or selective portal vein embolization. The protocol consisted of the following sequences: three-dimensional-encoded spoiled gradient-echo sequence (repetition time per echo time 15 per 2.7 ms, flip angle 20°) for volumetry, and two-dimensional-encoded time-of-flight angiography sequence (repetition time per echo time 18 per 6.4 ms, flip angle 80°) for vessel visualization. Liver volume and portal vein segmentation was performed using a dedicated postprocessing software. In animals with portal vein embolization, portography served as reference standard. True liver volume was measured after sacrificing the animals. Measurements were carried out by two independent observers with subsequent analysis by the Cohen κ-test for interobserver agreement.
MRI liver volumetry highly correlated with the true liver volume measurement using a conventional method in both the untreated liver and the liver remnant after 70% PH with a high interobserver correlation coefficient of 0.94 (95% confidence interval, 0.80-0.98 for untreated liver [P < 0.001] and 0.90-0.97 after 70% PH [P < 0.001]). The diagnostic accuracy of magnetic resonance angiography for the occlusion of one branch of the portal vein was 0.95 (95% confidence interval, 0.84-1). The level of agreement between the two observers for the description of intrahepatic vascular anatomy was excellent (Cohen κ value = 0.925).
This protocol may be used for noninvasive liver volumetry and visualization of portal vein anatomy in mice. It will serve the dynamic study of new strategies to enhance liver regeneration in vivo.

Keywords
Animals, Balloon Occlusion, Cell Differentiation, Hepatectomy, Hepatocytes/cytology, Liver/anatomy & histology, Liver/blood supply, Liver/physiology, Liver/surgery, Liver Regeneration, Magnetic Resonance Imaging/methods, Male, Mice, Mice, Inbred C57BL, Models, Animal, Organ Size, Portal Vein/anatomy & histology, Portal Vein/diagnostic imaging, Portal Vein/physiology, Portography
Pubmed
Create date
15/02/2017 11:40
Last modification date
20/08/2019 15:18
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