Sperm cryopreservation reduces offspring growth.

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Ressource 1Download: Nusbaumer et al. Proc. R. Soc. B 2019.pdf (2972.60 [Ko])
State: Public
Version: Author's accepted manuscript
License: Not specified
Serval ID
serval:BIB_61957805898A
Type
Article: article from journal or magazin.
Collection
Publications
Title
Sperm cryopreservation reduces offspring growth.
Journal
Proceedings. Biological sciences
Author(s)
Nusbaumer D., Marques da Cunha L., Wedekind C.
ISSN
1471-2954 (Electronic)
ISSN-L
0962-8452
Publication state
Published
Issued date
25/09/2019
Peer-reviewed
Oui
Volume
286
Number
1911
Pages
20191644
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Sperm cryopreservation is routinely used in reproductive medicine, livestock production and wildlife management. Its effect on offspring performance is often assumed to be negligible, but this still remains to be confirmed in well-controlled within-subject experiments. We use a vertebrate model that allows us to experimentally separate parental and environmental effects to test whether sperm cryopreservation influences offspring phenotype under stress and non-stress conditions, and whether such effects are male-specific. Wild brown trout (Salmo trutta) were stripped for their gametes, and a portion of each male's milt was cryopreserved. Then, 960 eggs were simultaneously fertilized with either non-cryopreserved or frozen-thawed semen and raised singly in the presence or absence of a pathogen. We found no significant effects of cryopreservation on fertilization rates, and no effects on growth, survival nor pathogen resistance during the embryo stage. However, fertilization by cryopreserved sperm led to significantly reduced larval growth after hatching. Males varied in genetic quality as determined from offspring performance, but effects of cryopreservation on larval growth were not male-specific. We conclude that cryopreservation causes a reduction in offspring growth that is easily overlooked because it only manifests itself at later developmental stages, when many other factors affect growth and survival too.
Keywords
sperm cryopreservation, salmonids, fish, brown trout, assisted reproductive technology, fish, good genes, infection, sperm cryopreservation, vertebrate
Pubmed
Funding(s)
Swiss National Science Foundation / Projects / 31003A_182265
Create date
06/09/2019 16:05
Last modification date
28/09/2019 6:08
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