The future key role of LC-high-resolution-MS analyses in clinical laboratories: a focus on quantification.

Details

Serval ID
serval:BIB_60F279CC74D8
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
The future key role of LC-high-resolution-MS analyses in clinical laboratories: a focus on quantification.
Journal
Bioanalysis
Author(s)
Rochat B., Kottelat E., McMullen J.
ISSN
1757-6199 (Electronic)
ISSN-L
1757-6180
Publication state
Published
Issued date
2012
Volume
4
Number
24
Pages
2939-2958
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish.
Abstract
For the last decade, high-resolution (HR)-MS has been associated with qualitative analyses while triple quadrupole MS has been associated with routine quantitative analyses. However, a shift of this paradigm is taking place: quantitative and qualitative analyses will be increasingly performed by HR-MS, and it will become the common 'language' for most mass spectrometrists. Most analyses will be performed by full-scan acquisitions recording 'all' ions entering the HR-MS with subsequent construction of narrow-width extracted-ion chromatograms. Ions will be available for absolute quantification, profiling and data mining. In parallel to quantification, metabotyping will be the next step in clinical LC-MS analyses because it should help in personalized medicine. This article is aimed to help analytical chemists who perform targeted quantitative acquisitions with triple quadrupole MS make the transition to quantitative and qualitative analyses using HR-MS. Guidelines for the acceptance criteria of mass accuracy and for the determination of mass extraction windows in quantitative analyses are proposed.
Pubmed
Web of science
Create date
21/02/2013 17:49
Last modification date
20/08/2019 14:18
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