Immunological heterogeneity of autoreactive T lymphocytes against the nicotinic acetylcholine receptor in myasthenic patients

Details

Serval ID
serval:BIB_60AF30B5AAF9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Immunological heterogeneity of autoreactive T lymphocytes against the nicotinic acetylcholine receptor in myasthenic patients
Journal
European Journal of Immunology
Author(s)
Zhang  Y., Schluep  M., Frutiger  S., Hughes  G. J., Jeannet  M., Steck  A., Barkas  T.
ISSN
0014-2980 (Print)
Publication state
Published
Issued date
12/1990
Volume
20
Number
12
Pages
2577-83
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Abstract
The response of human T lymphocytes against the nicotinic acetylcholine receptor (AChR) was studied in five patients with myasthenia gravis (MG) and in six healthy donors using either native Torpedo AChR or recombinant protein derived from the mammalian AChR alpha subunit (X4, residues 6-216 of mouse AChR alpha subunit). The present study demonstrates that (a) AChR-specific T helper cell lines can be generated from MG patients [either from peripheral blood lymphocytes (PBL) or from thymocytes] as well as from PBL of normal controls, (b) lymphocytes from MG patients, but not from controls, recognize the mammalian AChR but not the Torpedo receptor, (c) in humans, the HLA-DR2-associated T cell epitope is probably located in the region of residues 162-216 of the AChR alpha subunit and (d) there is a considerable heterogeneity of autoreactive T cell responses: (i) T cell lines from different HLA-type donors have distinct epitope profiles; (ii) the epitope specificity of the PBL-derived T cell line is different from that of the thymocyte-derived line; (iii) the epitope specificities of patient-derived T cell lines are different from those generated from normal controls who share the same HLA phenotype.
Keywords
Animals Cells, Cultured HLA Antigens/analysis Humans Lymphocyte Activation Mice Myasthenia Gravis/*immunology Peptide Fragments/immunology Receptors, Nicotinic/*immunology Recombinant Proteins/immunology Species Specificity T-Lymphocytes/*immunology Thymus Gland/cytology Torpedo
Pubmed
Web of science
Create date
25/01/2008 12:46
Last modification date
20/08/2019 14:18
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