Replication of the DNA A component of African cassava mosaic virus in a heterologous system

Details

Serval ID
serval:BIB_60AE1CF0136D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Replication of the DNA A component of African cassava mosaic virus in a heterologous system
Journal
Journal of General Virology
Author(s)
Paszkowski  U., Zhang  S. B., Potrykus  I., Paszkowski  J.
ISSN
0022-1317
Publication state
Published
Issued date
12/1993
Peer-reviewed
Oui
Volume
74 ( Pt 12)
Pages
2725-9
Notes
Comparative Study
Journal Article --- Old month value: Dec
Abstract
The capacity for autonomous replication of the DNA A of African cassava mosaic virus (ACMV), a member of the bipartite geminiviruses infecting dicotyledonous plants, has been compared in host and non-host cells. A derivative of the ACMV DNA A was transfected into tobacco and maize protoplasts. Although ACMV is not able to infect maize, replication of the DNA A in maize protoplasts was observed to occur. The efficiency of replication was 10 to 20% of that seen in tobacco protoplasts. In both plant systems, replication was detected after the onset of cell division. ACMV replication in maize cells was compared to that of wheat dwarf virus and found to be 10 to 20% of that observed with the monocotyledon-specific virus. Insertion of 1165 bp of non-viral DNA into the ACMV DNA A prevented replication in maize but not in tobacco.
Keywords
Blotting, Southern *DNA Replication DNA, Viral/*biosynthesis Geminiviridae/*genetics Mosaic Viruses/genetics/*growth & development Plants, Toxic Restriction Mapping Species Specificity Tobacco/microbiology Vegetables/*microbiology Virus Replication Zea mays/microbiology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 19:47
Last modification date
20/08/2019 14:18
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