The effect of cordycepin on the multiplication of Semliki Forest virus and on polyadenylation of viral RNA.

Details

Serval ID
serval:BIB_60ABD141E512
Type
Article: article from journal or magazin.
Collection
Publications
Title
The effect of cordycepin on the multiplication of Semliki Forest virus and on polyadenylation of viral RNA.
Journal
Archives of Virology
Author(s)
Wittek R., Koblet H., Menna A., Wyler R.
ISSN
0304-8608 (Print)
ISSN-L
0304-8608
Publication state
Published
Issued date
1977
Volume
54
Number
1-2
Pages
95-106
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Cordycepin (3'-deoxyadenosine), at a concentration of 20 microgram/ml, has a marked effect on Semliki Forest virus multiplication. The appearance of plaque forming units is delayed by about 2 hours and the yield greatly reduced. The incorporation of [3H] uridine into intracellular viral RNAs reaches less than 50 per cent of controls. However, no specific effect on poly (A) synthesis could be detected. The binding efficiency of viral RNAs on nitrocellulose membranes and poly (U) sepharose is not affected by cordycepin. The average poly (A) length of total intracellular viral RNA was calculated on the basis of the ratio of the radioactivity of adenosine-monophosphate: adenosine and found to be about 35 nucleotides in treated and untreated cells.
Keywords
Animals, Chick Embryo, Culture Techniques, Deoxyadenosines/pharmacology, Micropore Filters, Poly A/biosynthesis, Poly A/metabolism, RNA, Viral/biosynthesis, Semliki forest virus/drug effects, Semliki forest virus/growth & development, Virus Replication/drug effects
Pubmed
Web of science
Create date
24/01/2008 11:43
Last modification date
20/08/2019 15:18
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