Arginine, citrulline and nitrate concentrations in the cerebrospinal fluid from patients with acute hydrocephalus.
Details
Serval ID
serval:BIB_60A83DB9A138
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Arginine, citrulline and nitrate concentrations in the cerebrospinal fluid from patients with acute hydrocephalus.
Journal
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
ISSN
1570-0232 (Print)
ISSN-L
1570-0232
Publication state
Published
Issued date
15/05/2007
Peer-reviewed
Oui
Volume
851
Number
1-2
Pages
250-256
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
Citrulline and nitric oxide (NO) are synthesized by NO synthase (NOS) in a 1:1-stoichiometry. In this study, we determined by HPLC arginine and citrulline concentrations by fluorescence detection and nitrate levels by UV absorbance detection in the cerebrospinal fluid (CSF) from patients with acute hydrocephalus that underwent ventricular drainage. We found increased citrulline concentration (50.6+/-17.2 versus 20.9+/-2.0 microM) and decreased arginine/citrulline molar ratio (0.42+/-0.11 versus 1.12+/-0.16) in hydrocephalus patients, while arginine and nitrate concentrations and citrulline/nitrate molar ratio remained with little change. Citrulline has been determined as a marker of NOS activity in some studies, but it remains to be determined the extent at which this statement holds true, since other biochemical pathways also regulate the concentration of this amino acid. Our results suggest that citrulline is primarily synthesized from NOS in acute hydrocephalus. The evaluation of sample deproteinization by addition of methanol for the analysis of amino acids in CSF is also reported.
Keywords
Adult, Albumins/isolation & purification, Amino Acids/cerebrospinal fluid, Arginine/cerebrospinal fluid, Case-Control Studies, Citrulline/cerebrospinal fluid, Humans, Hydrocephalus/cerebrospinal fluid, Middle Aged, Nitrates/cerebrospinal fluid
Pubmed
Web of science
Create date
13/09/2019 12:24
Last modification date
15/10/2019 5:26