Screening the Impact of Sirtuin Inhibitors on Inflammatory and Innate Immune Responses of Macrophages and in a Mouse Model of Endotoxic Shock.

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State: Public
Version: author
Serval ID
serval:BIB_6089D37EA0E6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Screening the Impact of Sirtuin Inhibitors on Inflammatory and Innate Immune Responses of Macrophages and in a Mouse Model of Endotoxic Shock.
Journal
Methods in molecular biology
Author(s)
Ciarlo E., Roger T.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
1436
Pages
313-334
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The development and screening of pharmacological modulators of histone deacetylases (HDACs), and particularly sirtuins, is a promising field for the identification of new drugs susceptible to be used for treatment strategies in a large array of welfare-associated, autoimmune and oncologic diseases. Here we describe a comprehensive protocol to evaluate the impact of sirtuin-targeting drugs on inflammatory and innate immune responses in vitro and in a preclinical mouse model of endotoxemia. We first provide an overview on strategies to design in vitro experiments, then focus on the analysis of cytokine production by primary macrophages and RAW 267.7 macrophages at the mRNA and protein levels, and finally describe the setup and follow-up of a mouse model of inflammation-driven endotoxic shock.

Keywords
Animals, Cytokines/metabolism, Disease Models, Animal, Drug Evaluation, Preclinical, Gene Expression Regulation/drug effects, Histone Deacetylase Inhibitors/pharmacology, Immunity, Innate/drug effects, Macrophages/cytology, Macrophages/drug effects, Macrophages/immunology, Mice, RAW 264.7 Cells, Shock, Septic/drug therapy, Shock, Septic/immunology, Sirtuins/metabolism, Cytokine, Endotoxemia, Epigenetics, Histone deacetylases, Innate immunity, Macrophage, Pharmacological inhibitors, Sepsis, Sirtuins
Pubmed
Create date
02/06/2016 14:15
Last modification date
20/08/2019 14:17
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