Heterogeneity in the remodeling of aneurysms of the ascending aorta with tricuspid aortic valves.
Details
Serval ID
serval:BIB_5FBD80DBD011
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Heterogeneity in the remodeling of aneurysms of the ascending aorta with tricuspid aortic valves.
Journal
The Journal of thoracic and cardiovascular surgery
ISSN
1097-685X (Electronic)
ISSN-L
0022-5223
Publication state
Published
Issued date
11/2006
Peer-reviewed
Oui
Volume
132
Number
5
Pages
1010-1016
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
The study addresses mechanisms driving the formation of ascending aortic aneurysms by comparing the maximal dilatation area with the transition area immediately adjacent to the normal aortic tissue left in place during surgical repair.
Aortic wall specimens were taken from the maximal dilatation area and transition area in 10 patients undergoing surgery for ascending aortic aneurysms and fixed for histology and immunohistochemistry for vascular smooth muscle cells (alpha-actin), endothelial cells (CD31), and macrophages (CD68). Tissue concentrations of vascular endothelial growth factor, matrix metalloproteinase-2, and matrix metalloproteinase-9 were determined by enzyme-linked immunosorbent assay. The results are expressed as medians with their 25th and 75th centiles.
Vascular smooth muscle cells were significantly more abundant in the maximal dilatation area than in the transition area (20.3 [14.8-24.4]/10(-2) mm2 vs 8.0 [6.4-9.3]/10(-2) mm2, respectively, P = .002). In the maximal dilatation area, vascular smooth muscle cells had lost their typical lamellar organization, whereas it was preserved in the transition area. Microvessels were significantly more abundant in the media of transition area than in the maximal dilatation area (7.5 [2.9-10.1]/mm2 vs 1.75 [1.5-2.0]/mm2, respectively, P = .008) and were associated with an inflammatory cell infiltration that predominated in their immediate vicinity. There were no significant differences in vascular endothelial growth factor, matrix metalloproteinase-2, and matrix metalloproteinase-9 between both areas.
The transition area appears as a disease progression front characterized by microvessel formation and inflammatory cell infiltration. In contrast, increased vascular smooth muscle cell density in the maximal dilatation area suggests a healing process, although inefficient to prevent aortic dilatation.
Aortic wall specimens were taken from the maximal dilatation area and transition area in 10 patients undergoing surgery for ascending aortic aneurysms and fixed for histology and immunohistochemistry for vascular smooth muscle cells (alpha-actin), endothelial cells (CD31), and macrophages (CD68). Tissue concentrations of vascular endothelial growth factor, matrix metalloproteinase-2, and matrix metalloproteinase-9 were determined by enzyme-linked immunosorbent assay. The results are expressed as medians with their 25th and 75th centiles.
Vascular smooth muscle cells were significantly more abundant in the maximal dilatation area than in the transition area (20.3 [14.8-24.4]/10(-2) mm2 vs 8.0 [6.4-9.3]/10(-2) mm2, respectively, P = .002). In the maximal dilatation area, vascular smooth muscle cells had lost their typical lamellar organization, whereas it was preserved in the transition area. Microvessels were significantly more abundant in the media of transition area than in the maximal dilatation area (7.5 [2.9-10.1]/mm2 vs 1.75 [1.5-2.0]/mm2, respectively, P = .008) and were associated with an inflammatory cell infiltration that predominated in their immediate vicinity. There were no significant differences in vascular endothelial growth factor, matrix metalloproteinase-2, and matrix metalloproteinase-9 between both areas.
The transition area appears as a disease progression front characterized by microvessel formation and inflammatory cell infiltration. In contrast, increased vascular smooth muscle cell density in the maximal dilatation area suggests a healing process, although inefficient to prevent aortic dilatation.
Keywords
Actins/analysis, Aged, Antigens, CD/analysis, Antigens, Differentiation, Myelomonocytic/analysis, Aorta/pathology, Aortic Aneurysm/pathology, Aortic Valve, Dilatation, Pathologic, Disease Progression, Endothelial Cells/pathology, Enzyme-Linked Immunosorbent Assay, Female, Heart Valve Diseases/complications, Heart Valve Diseases/pathology, Humans, Macrophages/pathology, Male, Matrix Metalloproteinase 2/analysis, Matrix Metalloproteinase 9/analysis, Middle Aged, Myocytes, Smooth Muscle/pathology, Neovascularization, Pathologic, Platelet Endothelial Cell Adhesion Molecule-1/analysis, Vascular Endothelial Growth Factor A/analysis
Pubmed
Web of science
Open Access
Yes
Create date
30/03/2019 17:39
Last modification date
20/08/2019 14:17