High prevalence of secondary resistance mutations in Venezuelan HIV-1 isolates.

Details

Ressource 1Download: 16562642.pdf (509.41 [Ko])
State: Public
Version: author
Serval ID
serval:BIB_5F97D511348E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
High prevalence of secondary resistance mutations in Venezuelan HIV-1 isolates.
Journal
Investigacion clinica
Author(s)
Dieudonne M., Garzaro D., Torres J., Naranjo L., Suárez J.A., Castro J., Martínez N., Castro E., Berrueta L., Salmen S., Devesa M., Rangel H.R., Pujol F.H.
ISSN
0535-5133 (Print)
ISSN-L
0535-5133
Publication state
Published
Issued date
03/2006
Volume
47
Number
1
Pages
27-34
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The genetic variability was studied in HIV-1 from Venezuelan patients with and without treatment, in order to evaluate the presence of polymorphisms and drug resistance mutations. Proviral DNA from peripheral blood mononuclear cells or viral RNA from plasma was extracted from the blood of 30 patients. Two regions from the polymerase gene, protease (Pr) and reverse transcriptase (RT) and one genomic fragment from the envelope (Env) gene were amplified and sequenced. All HIV-1 samples analyzed were classified as subtype B, without evidence of recombination. Although no primary protease mutations were detected, a high frequency of secondary mutations (86%, 19/22), associated to restoration of viral replicative fitness, was observed in strains circulating both in treated and non-treated patients. Resistance mutations to nucleoside RT inhibitors (NRTI) and non-nucleoside RT inhibitors (NNRTI) were detected in 35% (6/17) and 12% (2/17) of the viruses circulating in treated patients, respectively. Resistance mutations were also present in the virus infecting one antiretroviral naive individual (7.7%), suggesting that local screening for resistant mutation in naive patient might be important to minimize therapy failure. Future studies are warranted to assess the role of secondary mutation in the success of viral infection.

Keywords
Adult, Drug Resistance, Viral, Female, HIV-1/drug effects, HIV-1/genetics, Humans, Male, Middle Aged, Mutation, Polymorphism, Genetic, Prevalence, Venezuela
Pubmed
Create date
04/05/2017 15:49
Last modification date
20/08/2019 14:17
Usage data