Effects of iron overload on transferrin secretion by cultured fetal rat hepatocytes.
Details
Serval ID
serval:BIB_5EFA6D3A5027
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Effects of iron overload on transferrin secretion by cultured fetal rat hepatocytes.
Journal
Biology of the Cell
ISSN
0248-4900[print], 0248-4900[linking]
Publication state
Published
Issued date
1989
Volume
65
Number
3
Pages
221-229
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
Primary cultures of fetal rat hepatocytes were maintained in an arginine-free medium deprived of serum but supplemented with 0.03 mM L-ornithine and 10(-8) M dexamethasone. Ferric nitrilotriacetate was added to the culture medium in order to obtain iron concentrations ranging from 10 to 100 microM. After 24 h of treatment, iron was visualized inside the hepatocytes by the staining method of Perls and electron microscope study. The present data demonstrate that iron overload decreases transferrin secretion; this effect appears not to be specific, since albumin production is affected in a similar manner. This depressed transferrin secretion is not the consequence of hepatocyte death, as the phenomenon is confirmed when expressed per cell. Since the corresponding mRNA is unaffected, it may be postulated that iron overload decreases transferrin secretion at some post-transcriptional level.
Keywords
Albumins/secretion, Animals, Cell Division, Cells, Cultured, DNA Replication/drug effects, Ferric Compounds, Iron/toxicity, Liver/cytology, Liver/metabolism, Nitrilotriacetic Acid/analogs &, derivatives, Rats, Rats, Inbred Strains, Time Factors, Transferrin/secretion
Pubmed
Web of science
Create date
24/01/2008 16:26
Last modification date
20/08/2019 15:16