Virosaurus A Reference to Explore and Capture Virus Genetic Diversity.

Details

Serval ID
serval:BIB_5E9093826570
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Virosaurus A Reference to Explore and Capture Virus Genetic Diversity.
Journal
Viruses
Author(s)
Gleizes A., Laubscher F., Guex N., Iseli C., Junier T., Cordey S., Fellay J., Xenarios I., Kaiser L., Mercier P.L.
ISSN
1999-4915 (Electronic)
ISSN-L
1999-4915
Publication state
Published
Issued date
01/11/2020
Peer-reviewed
Oui
Volume
12
Number
11
Pages
E1248
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
The huge genetic diversity of circulating viruses is a challenge for diagnostic assays for emerging or rare viral diseases. High-throughput technology offers a new opportunity to explore the global virome of patients without preconception about the culpable pathogens. It requires a solid reference dataset to be accurate. Virosaurus has been designed to offer a non-biased, automatized and annotated database for clinical metagenomics studies and diagnosis. Raw viral sequences have been extracted from GenBank, and cleaned up to remove potentially erroneous sequences. Complete sequences have been identified for all genera infecting vertebrates, plants and other eukaryotes (insect, fungus, etc.). To facilitate the analysis of clinically relevant viruses, we have annotated all sequences with official and common virus names, acronym, genotypes, and genomic features (linear, circular, DNA, RNA, etc.). Sequences have been clustered to remove redundancy at 90% or 98% identity. The analysis of clustering results reveals the state of the virus genetic landscape knowledge. Because herpes and poxviruses were under-represented in complete genomes considering their potential diversity in nature, we used genes instead of complete genomes for those in Virosaurus.
Keywords
HTS, bioinformatics, complete genome, database, diagnostics, sequencing, viral infections, viruses
Pubmed
Web of science
Open Access
Yes
Create date
09/11/2020 9:03
Last modification date
23/12/2020 6:24
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