Cell size as a determinant of the clone-forming ability of human keratinocytes

Details

Serval ID
serval:BIB_5E85AF9B9FAC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cell size as a determinant of the clone-forming ability of human keratinocytes
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Barrandon  Y., Green  H.
ISSN
0027-8424 (Print)
Publication state
Published
Issued date
08/1985
Volume
82
Number
16
Pages
5390-4
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Aug
Abstract
Keratinocytes isolated from human epidermis and subsequently cultured may form clones if they are 11 micron or less in diameter but are irreversibly committed to further enlargement and terminal differentiation if they are 12 micron or more in diameter. When a founding cell of 11 micron or less forms a small rapidly growing clone in culture, the cells of that clone are able to found new colonies even when their diameter is as great as 20 micron. As the clone becomes larger and grows more slowly, the maximal size of its clonogenic cells is reduced toward that of the epidermis. A cultured cell of up to 20 micron in diameter can, when it divides, give rise to clonogenic progeny smaller than itself, thus reversing the process of enlargement. Cells larger than 20 micron cannot divide and therefore cannot be rescued from terminal differentiation. It is concluded that when keratinocytes multiply rapidly, they extend reversibly the maximal size at which they are capable of generating clones into the range usually characteristic of terminally differentiating cells. It is proposed that this mechanism enables the keratinocyte to accommodate an increased rate of multiplication to its need to attain a large size during terminal differentiation.
Keywords
Cell Division Cells, Cultured Clone Cells Humans Infant, Newborn Keratins Kinetics Male Probability Skin/*cytology Time Factors
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 8:41
Last modification date
20/08/2019 14:16
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