Type VI Secretion System Substrates Are Transferred and Reused among Sister Cells.

Details

Serval ID
serval:BIB_5E70394B8769
Type
Article: article from journal or magazin.
Collection
Publications
Title
Type VI Secretion System Substrates Are Transferred and Reused among Sister Cells.
Journal
Cell
Author(s)
Vettiger A., Basler M.
ISSN
1097-4172 (Electronic)
ISSN-L
0092-8674
Publication state
Published
Issued date
22/09/2016
Peer-reviewed
Oui
Volume
167
Number
1
Pages
99-110.e12
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Bacterial type VI secretion system (T6SS) is a nanomachine that works similarly to a speargun. Rapid contraction of a sling (sheath) drives a long shaft (Hcp) with a sharp tip and associated effectors through the target cell membrane. We show that the amount and composition of the tip regulates initiation of full-length sheath assembly and low amount of available Hcp decreases sheath length. Importantly, we show that both tip and Hcp are exchanged by T6SS among by-standing cells within minutes of initial cell-cell contact. The translocated proteins are reused for new T6SS assemblies suggesting that tip and Hcp reach the cytosol of target cells. The efficiency of protein translocation depends on precise aiming of T6SS at the target cells. This interbacterial protein complementation can support T6SS activity in sister cells with blocked protein synthesis and also allows cooperation between strains to increase their potential to kill competition. VIDEO ABSTRACT.
Keywords
Bacterial Proteins/metabolism, Cytosol/metabolism, Genetic Complementation Test, Hemolysin Proteins/metabolism, Protein Transport, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/metabolism, Type VI Secretion Systems/metabolism, Vibrio cholerae/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
22/07/2024 16:10
Last modification date
27/07/2024 7:01
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