Investigating Candida glabrata Urinary Tract Infections (UTIs) in Mice Using Bioluminescence Imaging.

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License: CC BY 4.0
Serval ID
serval:BIB_5E410F94279E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Investigating Candida glabrata Urinary Tract Infections (UTIs) in Mice Using Bioluminescence Imaging.
Journal
Journal of fungi
Author(s)
Schrevens S., Sanglard D.
ISSN
2309-608X (Electronic)
ISSN-L
2309-608X
Publication state
Published
Issued date
09/10/2021
Peer-reviewed
Oui
Volume
7
Number
10
Pages
844
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Urinary tract infections (UTIs) are quite common and mainly caused by bacteria such as Escherichia coli. However, when patients have urinary catheters, fungal infections comprise up to 15% of these types of infections. Moreover, fungal UTIs have a high mortality, due to rapid spreading of the fungi to the kidneys. Most fungal UTIs are caused by Candida species, among which Candida albicans and Candida glabrata are the most common. C. glabrata is an opportunistic pathogenic yeast, phylogenetically quite close to Saccharomyces cerevisiae. Even though it is commonly isolated from the urinary tract and rapidly acquires resistance to antifungals, its pathogenesis has not been studied extensively in vivo. In vivo studies require high numbers of animals, which can be overcome by the use of non-invasive imaging tools. One such tool, bioluminescence imaging, has been used successfully to study different types of C. albicans infections. For C. glabrata, only biofilms on subcutaneously implanted catheters have been imaged using this tool. In this work, we investigated the progression of C. glabrata UTIs from the bladder to the kidneys and the spleen. Furthermore, we optimized expression of a red-shifted firefly luciferase in C. glabrata for in vivo use. We propose the first animal model using bioluminescence imaging to visualize C. glabrata in mouse tissues. Additionally, this UTI model can be used to monitor antifungal activity in vivo over time.
Keywords
Candida glabrata, bioluminescence, urinary tract infection
Pubmed
Web of science
Open Access
Yes
Create date
08/11/2021 10:42
Last modification date
20/11/2021 7:34
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