Isocitrate lyase in germinating Soybean

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State: Public
Version: author
Serval ID
serval:BIB_5E0C0CB86BBF
Type
Inproceedings: an article in a conference proceedings.
Publication sub-type
Abstract (Abstract): shot summary in a article that contain essentials elements presented during a scientific conference, lecture or from a poster.
Collection
Publications
Institution
Title
Isocitrate lyase in germinating Soybean
Title of the conference
16th Annual Meeting of the Union of Swiss Societies of Experimental Biology
Author(s)
Ruchti M., Widmer F.
Address
Zürich, Switzerland, March 29-30, 1984
ISBN
0014-4754
Publication state
Published
Issued date
1984
Peer-reviewed
Oui
Volume
40
Series
Experientia
Pages
612
Language
english
Abstract
In plant seeds, the action of the glyoxylate cycle is essential to the net synthesis of carbohydrates from reserve lipids. Isocitrate lyase (EC 4.1.3.1), which catalyses the cleavage of isocitrate into succinate and glyoxylate, is specific to this cycle. Its in vivo activity may be used as a direct measurement of the occurrence of this peculiar metabolic pathway. Using germinating soybean (Glycine max., var. Maple arrow), we present the evolution of isocitrate activity, which is best explainded by induction phenomena. Also, the enzyme from 5-day germinated cotyledons war purified using conventional techniques and various physico-chemical and reactional were determined. Its relative molecular mass is ca. 100 000, and the Km for isocitrate is equal to 1.75 mM.
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