HIV integration site selection: targeting in macrophages and the effects of different routes of viral entry

Details

Serval ID
serval:BIB_5DB3D91CB50B
Type
Article: article from journal or magazin.
Collection
Publications
Title
HIV integration site selection: targeting in macrophages and the effects of different routes of viral entry
Journal
Molecular Therapy
Author(s)
Barr  S. D., Ciuffi  A., Leipzig  J., Shinn  P., Ecker  J. R., Bushman  F. D.
ISSN
1525-0016
Publication state
Published
Issued date
08/2006
Peer-reviewed
Oui
Volume
14
Number
2
Pages
218-25
Notes
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't --- Old month value: Aug
Abstract
We have studied the selection of HIV DNA integration sites in primary macrophages to investigate two questions. First, mature macrophages do not divide, allowing us to investigate whether HIV integration targeting differs between dividing cells and nondividing cells. We sequenced and analyzed 754 unique integration sites and found that integration in macrophages is favored in active transcription units (TUs), as was observed previously for other cell types. However, HIV integration in genes was slightly less favored in macrophages than in dividing PBMC or T cell lines. Second, we compared integration targeting by HIV-vector particles bearing either of two different envelope proteins (HIV R5 Env or VSV-G) to determine whether the mechanism of entry influenced subsequent integration targeting. Integration sites generated by HIV R5- or VSV-G-bearing particles showed no significant differences in their distributions in the human genome. Analysis of additional published integration site sequences also indicated that the route of entry did not affect integration site selection for other viral envelopes as well.
Keywords
Base Sequence Computational Biology Genome, Human Granulocyte Macrophage Colony-Stimulating Factors, Recombinant HIV-1/*genetics Humans Macrophages/*virology Membrane Glycoproteins/physiology Receptors, HIV/physiology T-Lymphocytes/virology Transcription, Genetic Viral Envelope Proteins/physiology Virus Integration/*physiology
Pubmed
Web of science
Open Access
Yes
Create date
22/02/2008 15:45
Last modification date
20/08/2019 15:15
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