Oligomeric structure of the Bacillus subtilis cell division protein DivIVA determined by transmission electron microscopy.

Details

Serval ID
serval:BIB_5D55C7728A57
Type
Article: article from journal or magazin.
Collection
Publications
Title
Oligomeric structure of the Bacillus subtilis cell division protein DivIVA determined by transmission electron microscopy.
Journal
Molecular microbiology
Author(s)
Stahlberg H., Kutejová E., Muchová K., Gregorini M., Lustig A., Müller S.A., Olivieri V., Engel A., Wilkinson A.J., Barák I.
ISSN
0950-382X (Print)
ISSN-L
0950-382X
Publication state
Published
Issued date
06/2004
Peer-reviewed
Oui
Volume
52
Number
5
Pages
1281-1290
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
DivIVA from Bacillus subtilis is a bifunctional protein with distinct roles in cell division and sporulation. During vegetative growth, DivIVA regulates the activity of the MinCD complex, thus helping to direct cell division to the correct mid-cell position. DivIVA fulfils a quite different role during sporulation in B. subtilis when it directs the oriC region of the chromosome to the cell pole before asymmetric cell division. DivIVA is a 19.5 kDa protein with a large part of its structure predicted to form a tropomyosin-like alpha-helical coiled-coil. Here, we present a model for the quaternary structure of DivIVA, based on cryonegative stain transmission electron microscopy images. The purified protein appears as an elongated particle with lateral expansions at both ends producing a form that resembles a 'doggy-bone'. The particle mass estimated from these images agrees with the value of 145 kDa measured by analytical ultracentrifugation suggesting 6- to 8-mers. These DivIVA oligomers serve as building blocks in the formation of higher order assemblies giving rise to strings, wires and, finally, two-dimensional lattices in a time-dependent manner.
Keywords
Bacillus subtilis/chemistry, Bacterial Proteins/chemistry, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Bacterial Proteins/ultrastructure, Cell Cycle Proteins/chemistry, Cell Cycle Proteins/genetics, Cell Cycle Proteins/metabolism, Cell Cycle Proteins/ultrastructure, Microscopy, Electron, Models, Molecular, Protein Structure, Quaternary, Ultracentrifugation
Pubmed
Web of science
Create date
09/06/2023 16:04
Last modification date
28/07/2023 6:59
Usage data