Simultaneous determination of melatonin and 6-hydroxymelatonin in human overnight urine by LC-MS/MS.

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Version: Final published version
License: CC BY-NC-ND 4.0
Serval ID
serval:BIB_5CDFB8562D73
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Simultaneous determination of melatonin and 6-hydroxymelatonin in human overnight urine by LC-MS/MS.
Journal
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Author(s)
Magliocco G., Le Bloc'h F., Thomas A., Desmeules J., Daali Y.
ISSN
1873-376X (Electronic)
ISSN-L
1570-0232
Publication state
Published
Issued date
01/09/2021
Peer-reviewed
Oui
Volume
1181
Pages
122938
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
For the quantification of the pineal hormone melatonin and its metabolite, 6-hydroxymelatonin, in human overnight urine, a single accurate method by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed. Urine samples were deconjugated using β-glucuronidase/arylsulfatase from Helix pomatia before solid phase extraction (SPE) purification. Chromatographic separation was performed using a reverse phase C18 column with a 7-minute gradient elution. Water was used as matrix to prepare the calibration standards, and deuterated analogues of melatonin and 6-hydroxymelatonin were used as internal standards. This newly developed method was validated in terms of linearity, accuracy, repeatability, intermediate precision, recovery, matrix effect, and stability according to the guidelines of the European Medicines Agency. The method was successfully applied to the analysis of overnight urine samples from 12 healthy volunteers, showing significant correlations of urinary melatonin and 6-hydroxymelatonin excretion rates with age. The urinary 6-hydroxymelatonin to melatonin ratio was also established and will be assessed in further studies as a potential endogenous metric of CYP1A2 activity.
Keywords
CYP450, LC-MS/MS, Melatonin, Phenotyping
Pubmed
Web of science
Open Access
Yes
Create date
21/09/2021 11:30
Last modification date
12/02/2022 7:33
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