Polyhydroxyalkanoate synthesis in transgenic plants as a new tool to study carbon flow through beta-oxidation.

Details

Serval ID
serval:BIB_5CCC7E0E2363
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Polyhydroxyalkanoate synthesis in transgenic plants as a new tool to study carbon flow through beta-oxidation.
Journal
Plant Journal
Author(s)
Mittendorf V., Bongcam V., Allenbach L., Coullerez G., Martini N., Poirier Y.
ISSN
0960-7412 (Print)
ISSN-L
0960-7412
Publication state
Published
Issued date
1999
Volume
20
Number
1
Pages
45-55
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Transgenic plants producing peroxisomal polyhydroxy- alkanoate (PHA) from intermediates of fatty acid degradation were used to study carbon flow through the beta-oxidation cycle. Growth of transgenic plants in media containing fatty acids conjugated to Tween detergents resulted in an increased accumulation of PHA and incorporation into the polyester of monomers derived from the beta-oxidation of these fatty acids. Tween-laurate was a stronger inducer of beta-oxidation, as measured by acyl-CoA oxidase activity, and a more potent modulator of PHA quantity and monomer composition than Tween-oleate. Plants co-expressing a peroxisomal PHA synthase with a capryl-acyl carrier protein thioesterase from Cuphea lanceolata produced eightfold more PHA compared to plants expressing only the PHA synthase. PHA produced in double transgenic plants contained mainly saturated monomers ranging from 6 to 10 carbons, indicating an enhanced flow of capric acid towards beta-oxidation. Together, these results support the hypothesis that plant cells have mechanisms which sense levels of free or esterified unusual fatty acids, resulting in changes in the activity of the beta-oxidation cycle as well as removal and degradation of these unusual fatty acids through beta-oxidation. Such enhanced flow of fatty acids through beta-oxidation can be utilized to modulate the amount and composition of PHA produced in transgenic plants. Furthermore, synthesis of PHAs in plants can be used as a new tool to study the quality and relative quantity of the carbon flow through beta-oxidation as well as to analyse the degradation pathway of unusual fatty acids.
Keywords
Acyl-CoA Oxidase, Acyltransferases/metabolism, Angiosperms/genetics, Angiosperms/metabolism, Enzyme Activation, Fatty Acids/metabolism, Genes, Plant, Hydroxy Acids/metabolism, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Oxidation-Reduction, Oxidoreductases/metabolism, Peroxisomes/metabolism, Plants, Genetically Modified, Polysorbates, Thiolester Hydrolases/genetics, Thiolester Hydrolases/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 20:42
Last modification date
20/08/2019 15:15
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