Signals delivered via MHC class II molecules synergize with signals delivered via TCR/CD3 to cause proliferation and cytokine gene expression in T cells
Details
Serval ID
serval:BIB_5CA07AE9108D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Signals delivered via MHC class II molecules synergize with signals delivered via TCR/CD3 to cause proliferation and cytokine gene expression in T cells
Journal
Journal of Immunology
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
07/1992
Volume
149
Number
1
Pages
65-70
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jul 1
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jul 1
Abstract
We examined the role of MHC class II molecules in transducing signals to activated human T cells. Cross-linking of MHC class II molecules synergized with submitogenic amounts of anti-CD3 mAb in causing proliferation and secretion of the cytokines IL-2, IL-3, IFN-gamma, and TNF-alpha by MHC class II-alloreactive T cell lines. Signaling via MHC class II molecules in T cells resulted in activation of tyrosine kinases, in generation of inositol phosphates, and in Ca2+ mobilization that was abrogated by the tyrosine kinase inhibitor herbimycin A. Thus, like signaling via TCR/CD3, signaling via MHC class II molecules involved tyrosine kinase-dependent activation of phospholipase C, resulting in phosphoinositol turnover and Ca2+ flux. However the signaling pathways coupled to MHC class II molecules and to TCR/CD3 differed, because engagement of the transmembrane phosphatase CD45 inhibited Ca2+ fluxes triggered via TCR/CD3 but not Ca2+ fluxes triggered via MHC class II molecules.
Keywords
Antigens, CD/physiology
Antigens, CD3
Antigens, CD45
Antigens, Differentiation, T-Lymphocyte/*physiology
Benzoquinones
Calcium/metabolism
Cytokines/*genetics
Gene Expression
HLA-D Antigens/*physiology
Histocompatibility Antigens/physiology
Humans
Inositol Phosphates/metabolism
Lactams, Macrocyclic
*Lymphocyte Activation
Phosphotyrosine
Protein-Tyrosine Kinases/antagonists & inhibitors
Quinones/pharmacology
RNA, Messenger/genetics
Receptor Aggregation
Receptors, Antigen, T-Cell/*physiology
Second Messenger Systems
Signal Transduction
T-Lymphocytes/*physiology
Tyrosine/analogs & derivatives/metabolism
Pubmed
Web of science
Create date
25/01/2008 16:19
Last modification date
20/08/2019 15:15