Cloning and characterization of the genomic region encoding toxin IV-5 from the scorpion Tityus serrulatus Lutz and Mello.
Details
Serval ID
serval:BIB_5C8A8589BE17
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cloning and characterization of the genomic region encoding toxin IV-5 from the scorpion Tityus serrulatus Lutz and Mello.
Journal
Toxicon
ISSN
0041-0101 (Print)
ISSN-L
0041-0101
Publication state
Published
Issued date
1996
Peer-reviewed
Oui
Volume
34
Number
2
Pages
251-256
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
By means of PCR and using synthetic oligonucleotides designed from the reported cDNA, we amplified the gene that codes for toxin IV-5 from the Brazilian scorpion Tityus serrulatus. The analysis of the nucleotide sequence shows that the amplified genomic region is composed of 659 base pairs (bp) comprising two exons (28 and 284 bp) and an intron of 347 bp interrupting the region that encodes the signal peptide of the precursor toxin. Based on these findings a model for the structural organization of scorpion toxin genes is proposed.
Keywords
Amino Acid Sequence, Animals, Base Sequence, Blotting, Southern, Cloning, Molecular/methods, DNA/isolation & purification, Molecular Sequence Data, Polymerase Chain Reaction, Scorpion Venoms/chemistry, Scorpion Venoms/genetics, Scorpions/genetics
Pubmed
Web of science
Create date
10/03/2008 12:57
Last modification date
20/08/2019 15:15
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