Combined deletion of Glut1 and Glut3 impairs lung adenocarcinoma growth.

Details

Serval ID
serval:BIB_5C7C965ADC59
Type
Article: article from journal or magazin.
Collection
Publications
Title
Combined deletion of Glut1 and Glut3 impairs lung adenocarcinoma growth.
Journal
eLife
Author(s)
Contat C., Ancey P.B., Zangger N., Sabatino S., Pascual J., Escrig S., Jensen L., Goepfert C., Lanz B., Lepore M., Gruetter R., Rossier A., Berezowska S., Neppl C., Zlobec I., Clerc-Rosset S., Knott G.W., Rathmell J.C., Abel E.D., Meibom A., Meylan E.
ISSN
2050-084X (Electronic)
ISSN-L
2050-084X
Publication state
Published
Issued date
23/06/2020
Peer-reviewed
Oui
Volume
9
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Glucose utilization increases in tumors, a metabolic process that is observed clinically by <sup>18</sup> F-fluorodeoxyglucose positron emission tomography ( <sup>18</sup> F-FDG-PET). However, is increased glucose uptake important for tumor cells, and which transporters are implicated in vivo? In a genetically-engineered mouse model of lung adenocarcinoma, we show that the deletion of only one highly expressed glucose transporter, Glut1 or Glut3, in cancer cells does not impair tumor growth, whereas their combined loss diminishes tumor development. <sup>18</sup> F-FDG-PET analyses of tumors demonstrate that Glut1 and Glut3 loss decreases glucose uptake, which is mainly dependent on Glut1. Using <sup>13</sup> C-glucose tracing with correlated nanoscale secondary ion mass spectrometry (NanoSIMS) and electron microscopy, we also report the presence of lamellar body-like organelles in tumor cells accumulating glucose-derived biomass, depending partially on Glut1. Our results demonstrate the requirement for two glucose transporters in lung adenocarcinoma, the dual blockade of which could reach therapeutic responses not achieved by individual targeting.
Keywords
NanoSIMS, cancer biology, genetically engineered mouse model of cancer, glucose transporters, human, lamellar bodies, lung adenocarcinoma, mouse
Pubmed
Open Access
Yes
Create date
24/06/2020 13:03
Last modification date
30/06/2020 6:26
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