The CNGCb and CNGCd genes from Physcomitrella patens moss encode for thermosensory calcium channels responding to fluidity changes in the plasma membrane.

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Serval ID
serval:BIB_5C41FF658176
Type
Article: article from journal or magazin.
Collection
Publications
Title
The CNGCb and CNGCd genes from Physcomitrella patens moss encode for thermosensory calcium channels responding to fluidity changes in the plasma membrane.
Journal
Cell Stress and Chaperones
Author(s)
Finka A., Goloubinoff P.
ISSN
1466-1268 (Electronic)
ISSN-L
1355-8145
Publication state
Published
Issued date
2014
Volume
19
Number
1
Pages
83-90
Language
english
Abstract
Land plants need precise thermosensors to timely establish molecular defenses in anticipation of upcoming noxious heat waves. The plasma membrane-embedded cyclic nucleotide-gated Ca(2+) channels (CNGCs) can translate mild variations of membrane fluidity into an effective heat shock response, leading to the accumulation of heat shock proteins (HSP) that prevent heat damages in labile proteins and membranes. Here, we deleted by targeted mutagenesis the CNGCd gene in two Physcomitrella patens transgenic moss lines containing either the heat-inducible HSP-GUS reporter cassette or the constitutive UBI-Aequorin cassette. The stable CNGCd knockout mutation caused a hyper-thermosensitive moss phenotype, in which the heat-induced entry of apoplastic Ca(2+) and the cytosolic accumulation of GUS were triggered at lower temperatures than in wild type. The combined effects of an artificial membrane fluidizer and elevated temperatures suggested that the gene products of CNGCd and CNGCb are paralogous subunits of Ca(2+)channels acting as a sensitive proteolipid thermocouple. Depending on the rate of temperature increase, the duration and intensity of the heat priming preconditions, terrestrial plants may thus acquire an array of HSP-based thermotolerance mechanisms against upcoming, otherwise lethal, extreme heat waves.
Keywords
Plant heat shock response, Acquired thermotolerance, Ca2+ channels, Physcomitrella patens, Heat shock proteins
Pubmed
Web of science
Open Access
Yes
Create date
21/05/2013 14:14
Last modification date
20/08/2019 15:14
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