Quantification of 4 antidepressants and a metabolite by LC-MS for therapeutic drug monitoring.

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State: Public
Version: author
Serval ID
serval:BIB_5BEFDB40A967
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Quantification of 4 antidepressants and a metabolite by LC-MS for therapeutic drug monitoring.
Journal
Journal of Chromatography. B
Author(s)
Choong E., Rudaz S., Kottelat A., Haldemann S., Guillarme D., Veuthey J.L., Eap C.B.
ISSN
1873-376X (Electronic)
ISSN-L
1570-0232
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
879
Number
19
Pages
1544-1550
Language
english
Abstract
A liquid chromatography method coupled to mass spectrometry was developed for the quantification of bupropion, its metabolite hydroxy-bupropion, moclobemide, reboxetine and trazodone in human plasma. The validation of the analytical procedure was assessed according to Société Française des Sciences et Techniques Pharmaceutiques and the latest Food and Drug Administration guidelines. The sample preparation was performed with 0.5mL of plasma extracted on a cation-exchange solid phase 96-well plate. The separation was achieved in 14min on a C18 XBridge column (2.1mm×100mm, 3.5μm) using a 50mM ammonium acetate pH 9/acetonitrile mobile phase in gradient mode. The compounds of interest were analysed in the single ion monitoring mode on a single quadrupole mass spectrometer working in positive electrospray ionisation mode. Two ions were selected per molecule to increase the number of identification points and to avoid as much as possible any false positives. Since selectivity is always a critical point for routine therapeutic drug monitoring, more than sixty common comedications for the psychiatric population were tested. For each analyte, the analytical procedure was validated to cover the common range of concentrations measured in plasma samples: 1-400ng/mL for reboxetine and bupropion, 2-2000ng/mL for hydroxy-bupropion, moclobemide, and trazodone. For all investigated compounds, reliable performance in terms of accuracy, precision, trueness, recovery, selectivity and stability was obtained. One year after its implementation in a routine process, this method demonstrated a high robustness with accurate values over the wide concentration range commonly observed among a psychiatric population.
Pubmed
Web of science
Create date
20/06/2011 15:51
Last modification date
20/08/2019 15:14
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