Assessing dystrophies and other muscle diseases at the nanometer scale by atomic force microscopy.

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State: Public
Version: Final published version
Serval ID
serval:BIB_59BA463FB28E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Assessing dystrophies and other muscle diseases at the nanometer scale by atomic force microscopy.
Journal
Nanomedicine
Author(s)
van Zwieten R.W., Puttini S., Lekka M., Witz G., Gicquel-Zouida E., Richard I., Lobrinus J.A., Chevalley F., Brune H., Dietler G., Kulik A., Kuntzer T., Mermod N.
ISSN
1748-6963 (Electronic)
ISSN-L
1743-5889
Publication state
Published
Issued date
2014
Volume
9
Number
4
Pages
393-406
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
AIM: Atomic force microscopy nanoindentation of myofibers was used to assess and quantitatively diagnose muscular dystrophies from human patients.
MATERIALS & METHODS: Myofibers were probed from fresh or frozen muscle biopsies from human dystrophic patients and healthy volunteers, as well as mice models, and Young's modulus stiffness values were determined.
RESULTS: Fibers displaying abnormally low mechanical stability were detected in biopsies from patients affected by 11 distinct muscle diseases, and Young's modulus values were commensurate to the severity of the disease. Abnormal myofiber resistance was also observed from consulting patients whose muscle condition could not be detected or unambiguously diagnosed otherwise.
DISCUSSION & CONCLUSION: This study provides a proof-of-concept that atomic force microscopy yields a quantitative read-out of human muscle function from clinical biopsies, and that it may thereby complement current muscular dystrophy diagnosis.
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Web of science
Create date
30/07/2014 11:22
Last modification date
20/08/2019 15:13
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