The exon junction complex controls transposable element activity by ensuring faithful splicing of the piwi transcript.

Details

Serval ID
serval:BIB_597DB80469A3
Type
Article: article from journal or magazin.
Collection
Publications
Title
The exon junction complex controls transposable element activity by ensuring faithful splicing of the piwi transcript.
Journal
Genes & development
Author(s)
Malone C.D., Mestdagh C., Akhtar J., Kreim N., Deinhard P., Sachidanandam R., Treisman J., Roignant J.Y.
ISSN
1549-5477 (Electronic)
ISSN-L
0890-9369
Publication state
Published
Issued date
15/08/2014
Peer-reviewed
Oui
Volume
28
Number
16
Pages
1786-1799
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
Publication Status: ppublish
Abstract
The exon junction complex (EJC) is a highly conserved ribonucleoprotein complex that binds RNAs during splicing and remains associated with them following export to the cytoplasm. While the role of this complex in mRNA localization, translation, and degradation has been well characterized, its mechanism of action in splicing a subset of Drosophila and human transcripts remains to be elucidated. Here, we describe a novel function for the EJC and its splicing subunit, RnpS1, in preventing transposon accumulation in both Drosophila germline and surrounding somatic follicle cells. This function is mediated specifically through the control of piwi transcript splicing, where, in the absence of RnpS1, the fourth intron of piwi is retained. This intron contains a weak polypyrimidine tract that is sufficient to confer dependence on RnpS1. Finally, we demonstrate that RnpS1-dependent removal of this intron requires splicing of the flanking introns, suggesting a model in which the EJC facilitates the splicing of weak introns following its initial deposition at adjacent exon junctions. These data demonstrate a novel role for the EJC in regulating piwi intron excision and provide a mechanism for its function during splicing.
Keywords
Animals, Argonaute Proteins/genetics, Argonaute Proteins/metabolism, DNA Transposable Elements/physiology, DNA, Complementary/metabolism, Drosophila/genetics, Drosophila/metabolism, Drosophila Proteins/genetics, Drosophila Proteins/metabolism, Female, Gene Knockdown Techniques, Gene Silencing, Introns/genetics, Mutation, Ovary/cytology, Ovary/metabolism, Protein Subunits/metabolism, RNA Splicing, Ribonucleoproteins/genetics, Ribonucleoproteins/metabolism, AGO3, EJC, Piwi, piRNA, splicing, transposon
Pubmed
Web of science
Open Access
Yes
Create date
28/10/2019 12:57
Last modification date
29/10/2019 6:26
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