Fluorescein-labeled dextran concentration is increased in BAL fluid after ANTU-induced edema in rats

Details

Serval ID
serval:BIB_593CDF623973
Type
Article: article from journal or magazin.
Collection
Publications
Title
Fluorescein-labeled dextran concentration is increased in BAL fluid after ANTU-induced edema in rats
Journal
J Appl Physiol (1985)
Author(s)
Guery B. P., Nelson S., Viget N., Fialdes P., Summer W. R., Dobard E., Beaucaire G., Mason C. M.
ISSN
8750-7587 (Print)
ISSN-L
0161-7567
Publication state
Published
Issued date
09/1998
Volume
85
Number
3
Pages
842-8
Language
english
Notes
Guery, B P
Nelson, S
Viget, N
Fialdes, P
Summer, W R
Dobard, E
Beaucaire, G
Mason, C M
eng
Research Support, Non-U.S. Gov't
J Appl Physiol (1985). 1998 Sep;85(3):842-8. doi: 10.1152/jappl.1998.85.3.842.
Abstract
Several methodologies have been developed to assess alveolocapillary membrane permeability in acute lung injury. The purpose of this study was to determine the reliability of FITC-dextran compared with radioactive tracers to assess lung permeability alterations. After intraperitoneal administration of alpha-naphthylthiourea (ANTU, 50 mg/kg) or DMSO-ANTU vehicle, the animals were euthanized and their lungs were studied in an isolated-lung preparation. FITC-dextran or radiolabeled tracers were added to the perfusate. At 2 h the bronchoalveolar lavage (BAL) fluid from the ANTU group showed a significantly greater amount of fluorescence in the supernatant after centrifugation of BAL fluid compared with the DMSO group. Consistent results were observed with the radioactive tracers: there was an increase in extravascular albumin space and extravascular lung water compared with the control group. No cleavage of the FITC from the dextran molecule was evident by chromatography comparing samples recovered from the BAL fluid to the pure FITC-dextran molecule. In conclusion, measurement of FITC-dextran in the supernatant of BAL fluid after intravascular administration is a reliable method of assessing lung permeability changes in vivo and ex vivo.
Keywords
Animals, Bronchoalveolar Lavage Fluid/*chemistry, Capillary Permeability/physiology, Cell Count, Dextrans/*pharmacokinetics, Edema/chemically induced/*metabolism, Erythrocytes/metabolism, Fluorescein-5-isothiocyanate/*analogs & derivatives/pharmacokinetics, Injections, Intraperitoneal, Injections, Intravenous, Lung/metabolism, Pulmonary Circulation, Rats, Serum Albumin/metabolism, Thiourea/analogs & derivatives
Pubmed
Create date
29/04/2021 10:59
Last modification date
30/04/2021 6:38
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