Clonality of cold agglutinins in patients with hemolytic anemia: an analysis by high-resolution two-dimensional gel electrophoresis

Details

Serval ID
serval:BIB_58905E50882E
Type
Article: article from journal or magazin.
Publication sub-type
Case report (case report): feedback on an observation with a short commentary.
Collection
Publications
Institution
Title
Clonality of cold agglutinins in patients with hemolytic anemia: an analysis by high-resolution two-dimensional gel electrophoresis
Journal
American Journal of Hematology
Author(s)
Tissot  J. D., Clement  F., Schifferli  J. A., Frei  P. C., Hochstrasser  D. F., Schneider  P.
ISSN
0361-8609 (Print)
Publication state
Published
Issued date
07/1992
Volume
40
Number
3
Pages
171-5
Notes
Case Reports
Journal Article --- Old month value: Jul
Abstract
High-resolution two-dimensional gel electrophoresis (2-DGE) was used to analyse plasma samples and partially purified cold agglutinins (CA) obtained from two selected patients. Both presented an acute hemolytic anemia with CA of high thermal amplitude, normal immunoglobulin levels, no detectable paraproteinemia, and no clinical evidence of a malignant B-cell disorder. The electrophoretograms of their plasma showed evident alternations of the "normal" protein profile, which were directly related to hemolysis (absence of the spots of haptoglobin and in one case of those of hemopexin), but no monoclonal gammopathy. The electrophoretograms of their purified CA revealed two clearly different spot patterns respectively corresponding to a monoclonal IgM and to polyclonal IgM. These results show that the clonality of CA associated with hemolytic anemia can be easily determined by 2-DGE. This technique may be very useful to discriminate chronic cold agglutinin disease in the early phase from "parainfectious" CA.
Keywords
Adult Aged Agglutinins/*blood/isolation & purification Anemia, Hemolytic/*blood Cold Cryoglobulins Electrophoresis, Gel, Two-Dimensional Female Humans Immunoglobulin M/analysis Male Paraproteinemias/metabolism
Pubmed
Web of science
Create date
25/01/2008 15:34
Last modification date
20/08/2019 14:12
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